신세포암에서 RUNX3 비활성화와 병리학적 특성과의 관계
The Relationship between RUNX3 Inactivation and Its Pathological Features in Renal Cell Carcinoma
권휘안, 박철, 김은정, 하윤석, 김용준, 윤석중, 이상철, 김원재,
소속 상세정보
권휘안 ( Kwon Whi-An )
Chungbuk National University
박철 ( Park Cheol )
Chungbuk National University
김은정 ( Kim Eun-Jung )
Chungbuk National University
하윤석 ( Ha Yun-Sok )
Chungbuk National University
김용준 ( Kim Yong-June )
Chungbuk National University
윤석중 ( Yun Seok-Joong )
Chungbuk National University
이상철 ( Lee Sang-Cheol )
Chungbuk National University
김원재 ( Kim Wun-Jae )
Chungbuk National University
KMID : 0358320090500050432
Abstract
Purpose: DNA methylation is a key regulator of gene transcription and genomic stability, and alterations in DNA methylation are frequently detected in human tumors. Recent study has suggested that inactivation of runt-related transcription factor 3 (RUNX3), primarily epigenetic alterations in DNA methylation, is closely associated with bladder tumor stage, grade, and prognosis. The aim of this study was to evaluate the association between RUNX3 inactivation and renal cell carcinoma (RCC).
Materials and Methods: RCC tissues (n=56) were obtained from patients who underwent radical nephrectomy. The methylation pattern of RUNX3 was determined by using methylation specific-polymerase chain reaction (MS-PCR) and direct DNA sequencing.
Results: Methylation of the RUNX3 promoter was observed in 75.0% of the samples (42/56). The tumor stage and grade were significantly associated with the methylation status (p＜0.05, respectively). However, recurrence and progression of RCC were not significantly related to the methylation of the RUNX3 promoter region (log-rank test, p＞0.05, respectively).
Conclusions: This study demonstrated that promoter methylation of RUNX3 is frequently observed in RCC. In addition, RUNX3 methylation is closely associated with aggressive pathologic features.
키워드
Human RUNX3 protein;Methylation;Renal cell carcinoma
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