사춘기와 성년기 흰쥐에서 고환염전에 의한 대측고환 손상 차이에 대한 연구
The Different Effects of Testicular Torsion on the Contralateral Testis between Pubertal and Adult Rats
정성진, 최우석, 하승범, 정민수, 정재승, 백민기, 홍성규, 최황,
소속 상세정보
정성진 ( Jeong Seong-Jin )
분당서울대학교병원 비뇨기과
최우석 ( Choi Woo-Suk )
서울대학교 의과대학 비뇨기과학교실
하승범 ( Ha Seung-Beom )
서울대학교 의과대학 비뇨기과학교실
정민수 ( Jeong Min-Soo )
서울대학교 의과대학 비뇨기과학교실
정재승 ( Chung Jae-Seung )
포천중문의과대학 분당차병원 비뇨기과
백민기 ( Baek Min-ki )
건국대학교 의학전문대학원 비뇨기과학교실
홍성규 ( Hong Sung-Kyu )
서울대학교 의과대학 비뇨기과학교실
최황 ( Choi Hwang )
서울대학교 의과대학 비뇨기과학교실
KMID : 0358320090500070704
Abstract
Purpose: Our study aimed to determine whether the severity of damage to the contralateral testis by ipsilateral testicular torsion/detorsion in pubertal rats, which have an incomplete blood-testis barrier, is different from that in adult rats.
Materials and Methods: We divided pubertal (6 weeks, n=17) and adult (10 weeks, n=17) Sprague-Dawley (SD) rats into group S (sham; n=5), group O (orchiectomy; n=6), and group D (detorsion; n=6). After 4 hours’ torsion of the ipsilateral testis, we applied orchiectomy (group O) and detorsion (group D) depending on the group and compared the histopathologic changes and germ cell apoptosis of the contralateral testis at the age of 13 weeks.
Results: In each age group, increased interstitial area, edema, and germ cell sloughing were observed in group D. The mean seminiferous tubule diameter decreased more in group D than in group S or O in each age group (p＜0.05). The mean germ cell layer thickness and number of spermatids per tubule decreased more in group D than in group S or O in each age group; additionally, in group D, values decreased more in pubertal rats than in adult ones (p＜0.05, respectively). The mean numbers of terminal deoxyuridine nick-end labeling (TUNEL)-positive cells were less than 1.0 in groups S and O, which was smaller than in group D (p＜0.05); additionally, in group D, this value tended to be higher in pubertal rats than in adult ones (p=0.057).
Conclusions: SD rats with a detorsioned testis had more severe damage to the contralateral testis than did those undergoing orchiectomy of the torsioned testis. Also, when comparing the severity of damage to the contralateral testis after ipsilateral torsion/detorsion between pubertal and adult rats, rats at a pubertal age, when most testicular torsions occur in clinical situations, had more severe damage than did those at an adult age.
키워드
Spermatic cord torsion; Age factors; Testis
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