The Effect of the Partial Obstruction Site of the Renal Vein on Testis and Kidney in Rats: Is the Traditional Animal Model Suitable for Varicocele Research?
고기원, 김제종, 문두건, 김수웅, 윤덕기, 박재영, 채지윤,
소속 상세정보
고기원 ( Ko Ki-Won )
고려대학교 의과대학 비뇨기과학교실
김제종 ( Kim Je-Jong )
고려대학교 의과대학 비뇨기과학교실
문두건 ( Moon Du-Geon )
고려대학교 의과대학 비뇨기과학교실
김수웅 ( Kim Soo-Woong )
서울대학교 의과대학 비뇨기과학교실
윤덕기 ( Yoon Duck-Ki )
고려대학교 의과대학 비뇨기과학교실
박재영 ( Park Jae-Young )
고려대학교 의과대학 비뇨기과학교실
채지윤 ( Chae Ji-Yun )
고려대학교 의과대학 비뇨기과학교실
KMID : 0358320100510080565
Abstract
Purpose: We investigated the influence of the location of the partial renal vein obstruction on the left kidney, the bilateral testes, and cauda epididymal sperm quality and determined whether this animal model is suitable for varicocele study.
Materials and Methods: A total of 25 adult male Sprague-Dawley rats were assigned to three groups: group 1 (experimental varicocele by partial ligation medial to the internal spermatic vein for 8 weeks, n=8), group 2 (partial ligation lateral to the internal spermatic vein for 8 weeks, n=10), and group 3 (sham operation for 8 weeks, n=7). Rats in groups 1, 2, and 3 underwent a left nephrectomy and bilateral orchiectomy at 8 weeks after the operation. Histological changes and Johnsen score in both testes were analyzed. Fibrotic changes in the left kidney were assessed by quantitative image analysis. Numbers of sperm and proportions of motile sperm in the cauda epididymides were determined.
Results: Significant histological abnormalities and Johnsen score changes were observed in the testes in group 1. Renal fibrosis did not differ significantly among the groups. The proportions of motile sperm were significantly lower bilaterally in group 1 than in groups 2 and 3. However, the mean bilateral epididymal sperm count in group 1 was not significantly lower than in groups 2 and 3.
Conclusions: Our results showed that experimental varicocele in the rat, induced by partial ligation medial to the internal spermatic vein, influences epididymal sperm quality without harmful effects on the left kidney. The present study certifies that this traditional animal model is suitable for varicocele research.
키워드
Animal models;Physiopathology;Rats;Varicocele
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