Leptin Enhances Nitric Oxide-Dependent Relaxation of the Clitoral Corpus Cavernosum
이신영, 정우현, 이무열, 김세철, 김현우, 명순철,
소속 상세정보
이신영 ( Lee Shin-Young )
Seoul Medical Center Department of Urology
정우현 ( Chung Woo-Hyun )
Chung-Ang University College of Medicine Department of Urology and Advanced Urogenital Diseases Research Center
이무열 ( Lee Moo-Yeol )
Chung-Ang University College of Medicine Department of Physiology
김세철 ( Kim Sae-Chul )
Chung-Ang University College of Medicine Department of Urology and Advanced Urogenital Diseases Research Center
김현우 ( Kim Hyun-Woo )
Catholic University Medical Center Department of Urology
명순철 ( Myung Soon-Chul )
Chung-Ang University College of Medicine Department of Urology and Advanced Urogenital Diseases Research Center
KMID : 0358320110520020136
Abstract
Purpose: The effects of leptin on female sexual behaviors are controversial, and studies on this topic are limited. The objectives of this study were to evaluate the direct effects of leptin on clitoral vasoreactivity in vitro and to determine the mechanism of action.
Materials and Methods : Isometric tension studies were conducted to determine the effects of pretreatment with leptin (10-8 M) on the contractile responses of rabbit clitoral corpus cavernosal smooth muscle strips. The effects of leptin were assessed on precontraction induced by phenylephrine (PE; 10-9-10-4 M) and KCl (35-140 mM). We also examined the effect of leptin on relaxation induced by acetylcholine (ACh; 10-9-10-4 M), verapamil (10-10-10-6 M), and sodium nitroprusside (10-9-10-4 M) in PE-precontracted (10-5 M) strips.
Results: Leptin enhanced ACh-induced relaxation in PE-precontracted strips. L-NAME pretreatment significantly reduced the effect of leptin on ACh-induced relaxation, whereas L-arginine potentiated the effect of leptin. Leptin decreased the KCl-induced contractile responses. Leptin increased verapamil-induced relaxation responses. The relaxation effects of leptin on KCl-induced contraction were inhibited by 10-5 M methylene blue and L-NAME pretreatment.
Conclusions: A high concentration of leptin enhances ACh-dependent relaxation in clitoral cavernosal smooth muscles. These relaxation effects of leptin may occur through an NO-dependent mechanism and voltage-dependent calcium channel blockade.
키워드
Calcium channels; Clitoris; Leptin; Nitric oxide; Relaxation
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