Comparison of Human Muscle-Derived Stem Cells and Human Adipose-Derived Stem Cells in Neurogenic Trans-Differentiation
권은비, 이지영, Piao Shuyu, 김인걸, 라정찬, 이지열,
소속 상세정보
권은비 ( Kwon Eun-Bi )
Catholic University College of Medicine Department of Urology
이지영 ( Lee Ji-Young )
Catholic University College of Medicine Department of Urology
( Piao Shuyu )
Catholic University College of Medicine Department of Urology
김인걸 ( Kim In-Gul )
Catholic University College of Medicine Department of Urology
라정찬 ( Ra Jeong-Chan )
RNL Bio Co. Ltd. Stem Cell Research Center
이지열 ( Lee Ji-Youl )
Catholic University College of Medicine Department of Urology
KMID : 0358320110520120852
Abstract
Purpose: Erectile dysfunction (ED) remains a major complication from cavernous nerve injury during radical prostatectomy. Recently, stem cell treatment for ED has been widely reported. This study was conducted to investigate the availability, differentiation into functional cells, and potential of human muscle-derived stem cells (hMDSCs) and human adipose-derived stem cells (hADSCs) for ED treatment.
Materials and Methods: We compared the neural differentiation of hMDSCs and hADSCs. Human muscle and adipose tissues were digested with collagenase, followed by filtering and centrifugation. For neural induction, isolated hMDSCs and hADSCs were incubated in neurobasal media containing forskolin, laminin, basic-fibroblast growth factor, and epidermal growth factor for 5 days. Following neural induction, hMDSCs and hADSCs were differentiated into neural cells, including neurons and glia, in vitro.
Results: In neural differentiated hMDSCs (d-hMDSCs) and differentiated hADSCs (d-hADSCs), neural stem cell marker (nestin) showed a significant decrease by immunocytochemistry, and neuronal marker (β-tubulin III) and glial marker (GFAP) showed a significant increase, compared with primary hMDSCs and hADSCs. Real-time chain reaction analysis and Western blotting demonstrated significantly elevated levels of mRNA and protein of β-tubulin III and GFAP in d-hADSCs compared with d-hMDSCs.
Conclusions: We demonstrated that hMDSCs and hADSCs can be induced to undergo phenotypic and molecular changes consistent with neurons. The neural differentiation capacity of hADSCs was better than that of hMDSCs.
키워드
Adipose tissue; Cell differentiation; Muscles; Neurons; Stem cells
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