Tumor Establishment Features of Orthotopic Murine Bladder Cancer Models
이진석, 배민호, 최성호, 이상헌, 조영삼, 박흥재, Kwon Chil-Hun, 주관중,
소속 상세정보
이진석 ( Lee Jin-Seok )
Sungkyunkwan University School of Medicine Kangbuk Samsung Hospital Department of Urology
배민호 ( Bae Min-Ho )
Sungkyunkwan University School of Medicine Kangbuk Samsung Hospital Department of Urology
최성호 ( Choi Sung-Ho )
Sungkyunkwan University School of Medicine Kangbuk Samsung Hospital Department of Urology
이상헌 ( Lee Sang-Hun )
Sungkyunkwan University School of Medicine Kangbuk Samsung Hospital Department of Urology
조영삼 ( Cho Young-Sam )
Sungkyunkwan University School of Medicine Kangbuk Samsung Hospital Department of Urology
박흥재 ( Park Heung-Jae )
Sungkyunkwan University School of Medicine Kangbuk Samsung Hospital Department of Urology
( Kwon Chil-Hun )
Sungkyunkwan University School of Medicine Kangbuk Samsung Hospital Department of Urology
주관중 ( Joo Kwan-Joong )
Sungkyunkwan University School of Medicine Kangbuk Samsung Hospital Department of Urology
KMID : 0358320120530060396
Abstract
Purpose: Animal tumor models are important for the evaluation of novel therapeutic modalities. Since the initial report of an orthotopic bladder tumor model, several modifications have been proposed to improve the tumor take rate. Here we compared the HCl-pretreated and electrocauterization-pretreated orthotopic murine bladder tumor models.
Materials and Methods: MBT-2 murine bladder cancer cells were transurethrally implanted in the bladder of syngeneic C3H/He mice. The mice were divided into three groups according to pretreatment methods (electrocautery, HCl, and control group) and were subjected to pretreatment before instillation of MBT-2 tumor cells into the bladder. Mice were sacrificed on day 21, and bladders were harvested, weighed, and examined histopathologically.
Results: The tumor take rate of the control, electrocautery, and HCl groups was 0%, 54%, and 100%, respectively. The tumor take rate of the HCl group was significantly higher than that of the control group (p<0.01) and the electrocautery group (p=0.01). Pathologic reports revealed that all established bladder tumors were high-grade papillary urothelial carcinomas.
Conclusions: The HCl pretreatment model was a preferable murine bladder tumor model for evaluating further therapeutic interventions.
키워드
Animal models; Intravesical administration; Urinary bladder neoplasms
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