Synergistic Effect of Mesenchymal Stem Cells Infected with Recombinant Adenovirus Expressing Human BDNF on Erectile Function in a Rat Model of Cavernous Nerve Injury
김수진, 최세웅, 허경재, 박상훈, 성영철, Ha Y-Shin, 조혁진, 홍성후, 이지열, 황태곤, 김세웅,
소속 상세정보
김수진 ( Kim Su-Jin )
Catholic University School of Medicine Department of Urology
최세웅 ( Choi Sae-Woong )
Catholic University School of Medicine Department of Urology
허경재 ( Hur Kyung-Jae )
Catholic University School of Medicine Department of Urology
박상훈 ( Park Sang-Hoon )
Pohang University of Science & Technology Division of Molecular and Life Sciences
성영철 ( Sung Young-Chul )
Pohang University of Science & Technology Division of Molecular and Life Sciences
( Ha Y-Shin )
Catholic University School of Medicine Department of Urology
조혁진 ( )
Catholic University School of Medicine Department of Urology
홍성후 ( Hong Sung-Hoo )
Catholic University School of Medicine Department of Urology
이지열 ( Lee Ji-Youl )
Catholic University School of Medicine Department of Urology
황태곤 ( Hwang Tae-Kon )
Catholic University School of Medicine Department of Urology
김세웅 ( Kim Sae-Woong )
Catholic University College of Medicine Department of Urology
KMID : 0358320120530100726
Abstract
Purpose: To evaluate the combined role of mescenchymal stem cells (MSCs) infected with recombinant adenoviruses expressing human BDNF (rAd/hBDNF) on the erectile dysfunction in rat with cavernous nerve injury.
Materials and Methods: Rats divided into 4 groups: control group, bilateral cavernous nerve crushing group (BCNC group), BCNC with MSCs group and BCNC with MSCs infected with rAd/hBDNF group. After 4-week, functional assessment was done. PKH26 and BDNF staining of major pelvic ganglion and masson’s trichrome staining of corpus cavernosum were performed. Western blot analysis of endothelial nitric oxide synthase (eNOS) and neuronal nitric oxide synthase (nNOS) was done in corpus cavernosum.
Results: After 4 weeks, BCNC with MSCs and MSCs infected with rAd/hBDNF groups showed significantly well-preserved erectile function compared with BCNC group. Moreover, the erectile function of MSCs infected with rAd/hBDNF group was significantly well-preserved than BCNC with MSCs group. The smooth muscle of corpus cavernosum was significantly preserved in BCNC with MSCs and MSCs infected with rAd/hBDNF groups compared with BCNC group. More preservation of smooth muscle was observed in rats with MSCs infected with rAd/hBDNF than with MSCs alone. Significant increase expression of eNOS and nNOS was noted in rats with MSCs infected with rAd/hBDNF than with MSCs alone.
Conclusions: The erectile function was more preserved after injection with MSCs infected with rAd/hBDNF in rat with ED caused by cavernous nerve injury. Therefore, the use of MSC infected with rAd/hBDNF may have a better treatment effect on ED cause by cavernous nerve injury.
키워드
Brain-derived growth factor; Erectile dysfunction; Stem cells
원문 및 링크아웃 정보
등재저널 정보