Induction of Apoptosis of Bladder Cancer Cells by Zinc-Citrate Compound
홍성후, 최용선, 조혁진, 이지열, 황태곤, 김세웅,
소속 상세정보
홍성후 ( Hong Sung-Hoo )
Catholic University College of Medicine Department of Urology
최용선 ( Choi Yong-Sun )
Catholic University College of Medicine Department of Urology
조혁진 ( Cho Hyuk-Jin )
Catholic University College of Medicine Department of Urology
이지열 ( Lee Ji-Youl )
Catholic University College of Medicine Department of Urology
황태곤 ( Hwang Tae-Kon )
Catholic University College of Medicine Department of Urology
김세웅 ( Kim Sae-Woong )
Catholic University College of Medicine Department of Urology
KMID : 0358320120530110800
Abstract
Purpose: Zinc is one of the trace minerals in the body and is known to have an anticancer effect by inducing apoptosis in prostate cancer. We aimed to investigate the antiproliferative effects of a zinc-citrate compound in bladder cancer.
Materials and Methods: A bladder cancer cell line (MBT-2) was treated with a zinc-citrate compound at different time intervals and concentrations. Mitochondrial (m)-aconitase activity was determined by use of the aconitase assay. DNA laddering analysis was performed to investigate apoptosis of MBT-2 cells. The molecular mechanism of apoptosis was investigated by Western blot analysis of p53, p21waf1, Bcl-2, Bcl-xL, and Bax and also by caspase-3 activity analysis.
Results: Treatment with the zinc-citrate compound resulted in a time- and dose-dependent decrease in cell number of MBT-2 cells. M-aconitase activity was significantly decreased. DNA laddering analysis indicated apoptosis of MBT-2 cells. The zinc-citrate compound increased the expression of p21waf1 and p53 and reduced the expression of Bcl-2 and Bcl-xL proteins but induced expression of Bax protein. The zinc-citrate compound induced apoptosis of MBT-2 cells by activation of the caspase-3 pathway.
Conclusions: We have shown that a zinc-citrate compound induces apoptotic cell death in a bladder cancer cell line, MBT-2, by caspase-3 activation through up-regulation of apoptotic proteins and down-regulation of antiapoptotic proteins.
키워드
Apoptosis; Urinary bladder neoplasms
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