Expression of Aquaporin-3 in Ipsilateral Rat Kidney With Unilateral Partial Ureteral Obstruction
이지영, 신주현, 송기학, 임재성, 설종구,
소속 상세정보
이지영 ( Lee Ji-Young )
Chungnam National University School of Medicine Department of Urology
신주현 ( Shin Ju-Hyun )
Chungnam National University School of Medicine Department of Urology
송기학 ( Song Ki-Hak )
Chungnam National University School of Medicine Department of Urology
임재성 ( Lim Jae-Sung )
Chungnam National University School of Medicine Department of Urology
설종구 ( Sul Chong-Koo )
Chungnam National University School of Medicine Department of Urology
KMID : 0358320130540040266
Abstract
Purpose: Aquaporin (AQP), a protein located in the cellular membrane, allows rapid passage of water across the cell membrane. Various AQP subtypes have been associated with ureteral obstruction. In particular, AQP3 has two functions: water and glycerol transport. The aim of this study was to investigate the expression of AQP3 in the ipsilateral rat kidney in unilateral partial ureteral obstruction (UPUO).
Materials and Methods: Sprague-Dawley rats (n=30, 200-250 g) were divided into two groups. A sham operation was performed in the control group (n=10) and UPUO of the left upper ureter with a silicone tube was induced in the UPUO group (n=20). The left kidney was obtained from both groups 7 days after the operations. The kidney specimens underwent immunofluorescent staining with AQP3 monoclonal antibody, and the density of AQP3 in the tissue was measured with an image analyzer.
Results: In the UPUO group, thinning of the epithelial layer and infiltration of inflammatory cells was seen along with the localized expression of AQP3 in the basolateral aspect of the principal collecting duct cells. The mean optical density of AQP3 was significantly lower in the UPUO group than in the control group (100.9±17.5 compared with 131.7±16.9; p<0.001).
Conclusions: These results suggest that a decrease in the expression of AQP3 may be the result of a urinary stasis reaction caused by UPUO in response to local and intrarenal factors. These changes suggest that AQP3 may have a pathophysiological role in UPUO.
키워드
Aquaporin 3;Ureteral obstruction
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