Cell-Free microRNA-214 From Urine as a Biomarker for Non-Muscle-Invasive Bladder Cancer
김성민, 강호원, 김원태, 김용준, 윤석중, 이상철, 김원재,
소속 상세정보
김성민 ( Kim Sung-Min )
Chungbuk National University College of Medicine Department of Urology
강호원 ( Kang Ho-Won )
Chungbuk National University College of Medicine Department of Urology
김원태 ( Kim Won-Tae )
Chungbuk National University College of Medicine Department of Urology
김용준 ( Kim Yong-June )
Chungbuk National University College of Medicine Department of Urology
윤석중 ( Yun Seok-Joong )
Chungbuk National University College of Medicine Department of Urology
이상철 ( Lee Sang-Cheol )
Chungbuk National University College of Medicine Department of Urology
김원재 ( Kim Wun-Jae )
Chungbuk National University College of Medicine Department of Urology
KMID : 0358320130540110791
Abstract
Purpose: MicroRNAs are small noncoding RNAs and microRNA-214 (miR-214) has been associated with the inhibition of cancer cell growth, migration, and invasion. The aim of this study was to investigate whether cell-free miR-214 isolated from urine could be used as a biomarker for non-muscle-invasive bladder cancer (NMIBC).
Materials and Methods: A total of 138 patients with primary NMIBC and 144 healthy normal controls were enrolled in this study. By use of quantitative polymerase chain reaction (PCR), the urinary levels of cell-free miR-214 were measured and the clinicopathological parameters of patients with NMIBC were compared with those of the controls.
Results: The urinary levels of cell-free miR-214 were significantly higher in the NMIBC patients than in the controls (20.08±3.21 vs. 18.96±2.68, p=0.002). However, the urinary levels of cell-free miR-214 were neither graded nor staged for the NMIBC patients (p>0.05, each). When we compared the urinary levels of cell-free miR-214 according to clinical outcomes, patients with recurrence had lower levels of miR-214 than did those with no recurrence (19.24±2.67 vs. 20.41±3.41, p=0.023). By contrast, there were no significant differences in the urinary level of cell-free miR-214 between the NMIBC patients showing progression and those showing no progression (p=0.919). Multivariate Cox regression analysis revealed that urinary levels of cell-free miR-214 were an independent predictor of NMIBC recurrence (hazard ratio, 2.011; 95% confidence interval, 1.027 to 3.937; p=0.041).
Conclusions: Urinary levels of cell-free miR-214 could be an independent prognostic parameter for NMIBC recurrence. Thus, urinary cell-free microRNA-214 might be a useful prognostic marker for NMI BC.
키워드
MicroRNAs;Neoplasms;Recurrence;Urinary bladder
원문 및 링크아웃 정보
등재저널 정보