Effect of curcumin on the interaction between androgen receptor and Wnt/β-catenin in LNCaP xenografts
홍정희, 이길호, 최한용,
소속 상세정보
홍정희 ( Hong Jeong-Hee )
Dankook University College of Medicine Department of Urology
이길호 ( Lee Gil-Ho )
Dankook University College of Medicine Department of Urology
최한용 ( Choi Han-Yong )
Sungkyunkwan University School of Medicine Samsung Medical Center Department of Urology
KMID : 0358320150560090656
Abstract
Purpose: Curcumin is a nontoxic, chemopreventive agent possessing multifaceted functions. Our previous study showed that curcumin inhibits androgen receptor (AR) through modulation of Wnt/β-catenin signaling in LNCaP cells. Therefore, we investigated the in vivo effects of curcumin by using LNCaP xenografts.
Materials and Methods: LNCaP cells were subcutaneously inoculated in Balb/c nude mice. When the tumor volume reached greater than 100 mm3, either curcumin (500 mg/kg body weight) or vehicle was administered through oral gavage three times weekly for 4 weeks. The expression of AR and intermediate products of Wnt/β-catenin were assessed.
Results: Curcumin had an inhibitory effect on tumor growth during the early period, which was followed by a slow increase in growth over time. Tumor growth was delayed about 27% in the curcumin group. The mean prostate-specific antigen (PSA) doubling time in the curcumin group was approximately twice that in the untreated group. Curcumin significantly decreased AR expression at both the mRNA and protein level. The PSA levels tended to be reduced in the curcumin group. However, there were no significant changes in expression of Wnt/β-catenin pathway intermediates.
Conclusions: This study revealed that curcumin initially interferes with prostate cancer growth by inhibiting AR activity and possibly by reducing PSA expression. Further research is needed to investigate the plausible mechanism of the antiandrogenic action of curcumin.
키워드
Androgen receptors; Animal models; Curcumin; Prostate neoplasms
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