Ä¡¸éÂø»öÁ¦¸¦ ÀÌ¿ëÇÑ ±¤¿ªµ¿ Ä¡·áÀÇ Ç×±Õ È¿°ú
Antimicrobial Effect of Photodynamic Therapy Using Plaque Disclosing Agent
°íÁØÈñ, ¹ÚÈ£¿ø, ÀÌÁÖÇö, ¼Çö¿ì, À̽ÿµ,
¼Ò¼Ó »ó¼¼Á¤º¸
°íÁØÈñ ( Kho Jun-Hee )
Gangneung-Wonju National University College of Dentistry Department of Pediatric Dentistry
¹ÚÈ£¿ø ( Park Ho-Won )
Gangneung-Wonju National University College of Dentistry Department of Pediatric Dentistry
ÀÌÁÖÇö ( Lee Ju-Hyun )
Gangneung-Wonju National University College of Dentistry Department of Pediatric Dentistry
¼Çö¿ì ( Seo Hyun-Woo )
Gangneung-Wonju National University College of Dentistry Department of Pediatric Dentistry
À̽ÿµ ( Lee Si-Young )
Gangneung-Wonju National University College of Dentistry Department of Microbiology and Immunology
Abstract
ÀÌ ¿¬±¸ÀÇ ¸ñÀûÀº Ä¡¸éÂø»öÁ¦ÀÎ 10 - 20 mM erythrosineÀ» ±¤°¨°¢Á¦·Î »ç¿ëÇÑ ±¤¿ªµ¿ Ä¡·áÀÇ Ç×±Õ È¿°ú¸¦ Æò°¡ÇÏ´Â °ÍÀÌ´Ù. Streptococcus mutans, Lactobacillus casei, Candida albicans ¸¦ Æ÷ÇÔÇÏ´Â ´ÙÁ¾ ¿ì½Ä¿ø¼º ¼¼±Õ¸·À» hydroxyapatite disc¿¡ Çü¼ºÇÏ¿´´Ù. ±¤°¨°¢Á¦·Î 20 ¥ìM, 10 mM, 20 mM erythrosineÀ» 3ºÐ°£ Àû¿ë ÈÄ 24ÃÊ µ¿¾È ±¤Á¶»ç¸¦ ½ÃÇàÇÏ¿´´Ù. °¢ ½ÇÇ豺ÀÇ Colony-forming unit (CFU)À» ÃøÁ¤ÇÏ°í ¼¼±Õ¸·À» Confocal laser scanning microscopy (CLSM)À» ÀÌ¿ëÇÏ¿© °üÂûÇÏ¿´´Ù. 10 - 20 mM erythrosineÀ» ±¤°¨°¢Á¦·Î »ç¿ëÇÑ ±¤¿ªµ¿ Ä¡·á ½ÇÇ豺¿¡¼ CFUÀÇ ÇöÀúÇÑ °¨¼Ò°¡ °üÂûµÇ¾ú°í, ±× °á°ú´Â CLSM °üÂû¿¡¼µµ È®ÀεǾú´Ù. À̹ø ¿¬±¸¸¦ ÅëÇØ Ä¡°ú ÀÓ»ó¿¡¼ »ç¿ëµÇ´Â Ä¡¸éÂø»öÁ¦¸¦ ±¤°¨°¢Á¦·Î ÀÌ¿ëÇÑ ±¤¿ªµ¿ Ä¡·áÀÇ ³ôÀº Ç×±ÕÈ¿°ú¸¦ È®ÀÎÇÒ ¼ö ÀÖ¾ú´Ù.
The aim of this study was to evaluate the antimicrobial effect of photodynamic therapy (PDT) using plaque disclosing agent, 10 - 20 mM erythrosine, as a photosensitizer. Multispecies cariogenic biofilms containing Streptococcus mutans , Lactobacillus casei and Candida albicans were formed on hydroxyapatite disc. 20 ¥ìM, 10 mM and 20 mM erythrosine were applied as a photosensitizer for 3 minutes, and then light-emitting diode (LED) irradiated for 24 seconds. Colony-forming unit (CFU) were measured and biofilms were observed using confocal laser scanning microscopy (CLSM). CFU were significantly decreased in the PDT groups using 10 - 20 mM erythrosine (10 mM, 20mM) and the results were also confirmed by CLSM. This study confirms the high antimicrobial effect of photodynamic therapy using plaque disclosing agent as a photosensitizer.
Å°¿öµå
Photodynamic therapy; Erythrosine; Plaque disclosing agent; Multispecies biofilm
¿ø¹® ¹× ¸µÅ©¾Æ¿ô Á¤º¸
µîÀçÀú³Î Á¤º¸