일반시설에서 사육되는 마우스의 품질향상을 위한 기초조사 연구
Survey on environmental condition and health state of laboratory mouse in conventional facility
김경진, 김철규, 성노현, 이흥식,
소속 상세정보
김경진 ( )
서울대학교 생명과학부
김철규 ( )
국립독성연구소 병리부
성노현 ( )
서울대학교 생명과학부
이흥식 ( )
서울대학교 수의과대학 및
KMID : 0359320000400030611
Abstract
For the improvement of quality control of laboratory mouse, we investigated the environmental condition, histopathological findings and serological test using ELISA to mouse hepatitis virus(MHV), Mycoplasma pulmonis(MP), Clostridium piliforme(TZ) and Sendai virus (HVJ) of ICR, C57BL/6, CBA and C3H/He mice that were supplied from conventional laboratory animal facility.
1. The ammonia concentration of facility was below the recommended concentration, 15ppm, by the KNIH, and the room temperature(21∼23℃) and relative humidity(40∼60%) was optimum range recommend by the Ministry of Health and Welfare, respectively.
2. The incidence rate of inapparent disease was 86.6% and the major findings in the liver were vacuolar degeneration with nucleic pleomorphism. The lung was shown the thickening of alveolar wall and interstitial pneumonia with congestion. The kidney and spleen were observed the mild congestion and extramedullary hematopoiesis, respectively.
3. The positive reaction rates against MHV and MP in serological test was 97.9% and 37.5%, respectively but HVJ and TZ were negative.
These results suggest that laboratory mice could be infected with MHV and MP under conventional environments. Therefore we recommend to select thoroughly inapparent infected mice and to convert conventional system into SPF facility as soon as possible.
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