Ca++이 초기계태의 신경배형성의 미치는 영향
The Effect of Ca++ on Neurulation of Early Chick Embryos
김동호, 김영규, 이희봉, 이현구,
소속 상세정보
김동호 ( )
Chungbuk National University
김영규 ( )
Chungbuk National University
이희봉 ( )
Chungbuk National University
이현구 ( )
Chungbuk National University
KMID : 0359819930220020280
Abstract
Chick embryos have been used widely as model systems for studies in experimental embryology and teratology. Especially early chick embryos are very useful for studies of neural tube defects.
We made a try to figure out the effect of Ca++ on neurulation using Ca++ antagonist and Ca++ free media in early chick embryo, which is stage 8 by Hamburger & Hamilton classification. After 6~9 hours incubation in media treated with verapamil and
media
without Ca++, we observed the effects of these agents by LM and EM.
All of 12 embryos incubated in Ca++ free media showed that neural tube was diffusely opened along the whole axis and other deformity was not found. The chick embryos cultured in medium treated with verapamil revealed various abnormalities in dose
related manner. The case of arrest of the growth was also observed, which was not found in Ca++ free media group.
On scanning electron microscopic examination, the surface of neuroepithelium was flattened in embryos cultured in Ca++ free media.
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