유전적으로 변이된 흰쥐 섬유아세포에 의한 L-dopa 및 도파민의 실험관내 분비
In Vitro Release of L-dopa and Dopamine by Genetically Modified Fibroblasts of the Rat
정봉섭,
소속 상세정보
정봉섭 ( )
한림대학교 의과대학 부속강남성모병원
KMID : 0359819930220040529
Abstract
A construct DNA containing the cDNA for rat tyrosine hydroxylase (TH) and another construct DNA containing the cDNA for bovine L-dopa decarboxylase (DDC) were made and those genes were transferred by calcium phosphate transfection method into the
immortalized rat fibroblasts. A both TH-& DDC-positive clone was identified by immunocytochemical staining. These cells produced L-dopa and dopamine and released them into the cell culture medium in vitro, which was confirmed by high performance
liquid
chromatography. These results have general implications for the application of gene therapy and specific implications for Parkinson disease.
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