수산화도파민으로 유발된 파킨슨씨병 모델 흰쥐의 행동학 및 면역조직화학적 특성에 관한 연구
Behavioral and Immunohistochemical Characterization of 6-Hydroxydopamine Induced Rat Models of Parkinson's Disease
소속 상세정보
장웅규/Ung Kyu Chang
정천기/오창완/한대희/김현집/조자선/김용식/박찬웅/Chun Kee Chung/Chang Wan Oh/Dae Heo Han/Byun Jib Kim/Sa Sun Cho/Yong Sik Kim/Chan Woon Park
KMID : 0359819980270020139
Abstract
결론
1) Brundin 전파괴 모델, Perese 전파괴 모델, 부분파괴모델 등 3가지 모델 중 Perese 전파
괴 모델이 높은 성공적 병소제작율을 보였다
2) Amphetamine을 이용한 회전반응 결과는 hydroxylase 면역조직화학염색 소견과 잘 일치
하였으나 apomorphine을 이용한 회전반응의 결과는 조직염색과 일치하지 않았다
#초록#
There are several methods with which 6-hydroxy-dopamine is injected into the
nigrostriatal pathway in rats for making models of Parkinson's disease. One is a
complete lesion model in which A9 and A10 dopamine cells are destroyed, and the other
one is a partial lesion model in which only A9 dopamine cells are destroyed. The aim of
this study is to establish the model most suitable fro transplantation of neural tissue.
First, the behavioral change was investigated after dopamine releasing(amphetamine)
or dopamine agonist(apomorphine) substances were injected. And then,
immunohistochemical staining for tyrosine hydroxylase(TH) of the striatum and the
substania nigra was performed. Sixteen complete lesion models of Brundin, 4 complete
lesion models of Perese, and 5 partial lesion models of Perese were made. The rotation
response to amphetamine injection(5mg/kg, intraperitoneally) was checked 2 weeks after
lesion making. For 6 rats, which showed rotation more than 7 turns/minute with
amphetamine, the rotation response to subcutaneous injection of apomorphine was
examined.
Five complete lesion model of Brudin, 1 partial lesion model of Perese and 4 complete
lesion model of Perese demonstrated rotation above 7 turns/minute in amphetamine test.
Immunohistochemical staining of substantia nigra and corpus striatum for TH was faint
on the lesioned side in rats which showed rotation above 7 turns/min in
amphetaine-induced rotation test. irrespective of the kinds of model, while those areas of
the normal side showed dense staining for TH. However, the results of
immunohistochemical staining did not coincide with the results of rotation test by
apomorphine.
키워드
Parkinson's disease; Animal model; 6-hydroxydopamine rotation test; immunohistochemical staining;
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