냉농 보관제의 농도 변화가 신경 세포 생존율에 비치는 영향
The Influence of Different Concentrations of Cryoprotectants on Neuronal Cell Viability
소속 상세정보
김영수/Young Soo Kim
한기수/이언/김영보/유영미/Ki Soo Han/Uhn Lee/Young Bo Kim/Young Mi Yoo
KMID : 0359819980270030299
Abstract
결론
백서 중뇌 조직의 냉동보관 실험에서 냉동보관제의 농도를 변화시켜 세포 생존율을 측정
하였고 다음과 같은 결론을 얻었다
1) 냉동 보관제의 농도가 DMSO가 10∼15%일 때 FBS가 10∼50%인 경우 서로 비슷하게
70% 이상의 세포 생존율을 보였다.
2) 통계적으로 유의한 가장 높은 세포 생존율을 보인 것은 group H(76%)와 group D(75%)
였다
3) 냉동 보관제의 농도가 가장 높은 것보다는, 적절한 농도의 조합일 때 가장 좋은 세포 생
존율을 얻을 수 있었다.
4) 냉동 및 해동의 조건을 잘 맞추어주고. 적절한 냉동보관제를 사용할 경우에 냉동 보관
기법은 미성숙 뇌 조직의 보관 및 저장에 실제적으로 유용한 방법으로 사료된다.
#초록#
A promising technique for the treatment of Parkinson's disease and other various
neurodegenerative disorders is the transplantation of fetal neural tissue. There must,
however, be a prompt and reliable source, and one solution is cryopresevation, where
tissue viability can maintained for prolonged periods. Fetal neural tissue is, however,
known to be susceptible to freeze-storage damage during cryopresevation. In this study,
we examined the influence of different concentrations of cryoprotectants upon the
survival of rat fetal neurones. Fetal rat brain tissues was frozen with 7-15% dimenthly
sulfoxide(DMSO) and 10-50% fetal bovine serum(FBS) as cryoprotectants, then stored
for a period of 5 months. Post-storage neuronal cell viability was assessed by vital
staining followed by determination of cell density. Average total viability of frozen cells
with 7% DMSO and 10-50% FBS was less than 50%. Cryopreserved cells with 10-50%
DMSO and 10-50% FBS showed almost the same viability(around 70%). The highest
viability was obtained with 15% DMSO＋20% FBS combination(76%) and 10% DMSO＋
10% FBS combination(75%). Theoretically, the higher the concentration of
cryoprotectants, the higher the viability ; however, the best result was achieved stated
above, when the combination of cryoprotectants was at the concentrations statede above.
키워드
Rat fetal neural tissue; Cryopreservation; Cell viability; Cryoprotectant.;
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