일괄성 뇌허혈후 Amyloid Precursor Protein mRNA와 Amyloid Precursor Protein 발현의 변화
The Change of Expression in Amyloid Precursor Protein mRNA and Amyloid Precursor Protein by Transient Ischemia
소속 상세정보
이상형/Sang Hyung Lee
김혜선/정성진/이영재/서유헌/양희진/정영섭/한대희/조병규/Hye Sun Kim/Sung Jin Jeong/Young-Jae Lee/Yoo Hun Suh/Hee Jin Yang/Young Seob Jung/Dar Hee Han/Byung-Kyu Cho
KMID : 0359819980270111481
Abstract
결 론
본 연구의 결과는 1시간의 중대뇌동맥의 폐색 후 재관류시켰을때 백서의 대뇌피질에서
APPmRNA의 주된 3 이성형인 APP770. APP751, APP695의 mRNA의 변화가 유도되었다.
첫째, APP751 및 APP770의 mRNA는 1일 이후에 증가하기 시작하여 3일째 최고 수준으로
증가하였으며, 7일째에는 다시 재관류하기 전으로 감소하였다. APP770의 mRNA의 증가는
1일째에는 수술하기 전과 단순히 폐색시킨 것과 비교할 때 증가의 차이가 있었으며(p<0.01),
3일째에는 APP770 및 APP751의 myNA의 증가가 모두 통계적으로 유의하였다(p<0.01). 둘
째, APP695의mRNA는 1일, 3일째에는 감소하는 것으로 보였으며 수술 하기 전과 비교할
때 3일째에는 유의한 감소가 있었다(p<0.05).
일과성 뇌허혈로 대뇌피질에서의 APP는 재관류후 7일까지 변화가 없는 것으로 나타났다.
따라서 APP는 KPI-APP가 많이 생겼을 것이므로 아직 그 생리적인 기능은 정확히 알지 못
하지만 허혈 후 대사에 KPI-APF가 영향을 미칠 것으로 생각되므로 그 역할에 대하여 더
많은 연구가 필요할 것으로 생각되었다.
#초록#
Although the mechanism has not been clearly understood, it has been reported that
various altered gene expressions are induced by cerebral ischmia. In order to investigate
the effects of transient cerebral ischemia on the amyloid precursor protein(APP)
metabolism, the 3 isoforms of APP mRNA and the APP were examined.
Rats were given inchemic insult through middle cerebral artery occlusion and
reperfusions using blunted 4-0 nylon thread after exposure of cervical region. Group
were assigned with each being 1 hour occlusion and 1hr, 2hrs, 8hrs, 1 day, 3days, 7days
after reperfusion. The rats were then sacrificed and cerebral cortex of each animal was
dissected. The mRNA level of APP770, 751, 695 was investigated by reverse
transcription coupled polymerase chain reaction (RT-PCR)and the protein amount of was
investigated by Western blotting method.
The results showed that transient Ischemia induced the change of APP mRNA The
APP mRNA which encodes the KPI(Kunitz-type protease inhibitor) domain began to
increase after 1 day. reaching a maximun at 3 days and then decreased to control level
in the 7 days The protein level of APP was same until 7 days.
We Conclude that transient cerebral ischemia alters the gene expression of APP
isoforms. Therefore. we speculate that some factors regulating the splicing of APP gene
transcript may also undergo ischemia-induced changes and the increase in levels of
KPI-APP following ischemia might be associated with pathological changes during the
ischemic process.
Transient cerebral ischemia; Middle cerebral artery; APP(amyoid precursor protein); KPI(Kunitz-Type protease inhibitor);
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