Chloride Channel이 방수생성에 미치는 영향
The Effect of Chloride Channel on Aqueous Humor Production
대한안과학회지 1997년 38권 12호 p.2167 ~ 2171
소속 상세정보
기창원/Chang Won Kee
KMID : 0360219970380122167
Abstract
-요약-
세포의 용적을 조절하는 것은 상피세포의 분비기능에 있어 중요한 인자이며 regulatory
volume decrease(RVD) 현상이 관여하는 것으로 알려져 있다. 그리고 RVD에
K+ 와 Cl- channel이 관여하고 있다는 것이 알려져 있으며 모
양체에도 이런 channel이 존재하는 것이 밝혀져 있다. 그러므로 저자는 Cl channel이 방수
생성에 영향을 미치는지를 관찰하기 위해 chloride channel 차단제인 DIDS를 주입후 방수생
성의 변화를 관찰하였다. 체중 2.0-2.5kg의 백색가토에 한쪽 눈은 유리체로 2×
10-4M의 DIDS(4.4-diisothiocyanatostilbene-2-2-disulfonic acid)를
microsyringe를 이용해 주입하고 반대편 눈에는 생리식염수를 같은 방법으로 주입한 후 안
압측정과 fluorophotometer를 사용하여 양안의 방수생성율을 측정하고 Wilcoxon's signed
rank test로 통계처리하였다. DIDS에 의해 안압은 12.5%(P<0.05)의 감소를 보였으며 방수생
성율에서도 28.5%(P<0.05)의 감소를 보였다. 그러므로 가토모양체의 방수생성에 chloride
channel이 관여함을 관찰할 수 있었다.
#초록#
Regulating the cell volume is an important factor in secretory function of epithelial
cells and regulatory volume decrease(RVD) phenomenon is involved in response to the
changes of cell volume and osmolarity. RVD of epithelial cells reflects cellular release of
K+ and Cl- through channels and K+and
Cl- channels were verified in the basal membrane of the non pigmented
ciliary epithelial cells. Therefore, we attempted to observe the involvement of
Cl- channel in aqueous humor production by performing the
fluorophotometry after administration of the DIDS (4.4-diisothiocyan
atostilbene-2-2-disulfonic acid), the Cl channel blocker.
Ten white New Zealand rabbits, 5 for tonometry and 5 for fluorophotometry, were
used. One eye was injected 2×10-4M DIDS intravitreally using
microsyringe and the other eye was injected normal saline as a control in each rabbits.
Tonometry was performed before the injection and every hour after dosing for 5 hours.
Fluorophotometry was performed every 30 minutes for 3 hours starting 2 hours after
injection. Wilcoxon's signed rank test was used for statistical analysis.
DIDS decreased intraocular pressure by 12.5%(P<0.05) and reduced aqueous humor
flow rate by 28.5%(P<0.05). In conclusion, it was observed that the Cl-
channel influences the aqueous humor production in rabbit.
키워드
Regulatory volume decrease; Cl channel; DIDS; Aqueous humor production;
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