암세포응집소의 항암작용에 관한 실험적 연구 ( Purification of Agglutinin from Piellia tumberg and Its Cytotoxic Activity on Murine Leukemic Lymphoblast L5178Y )
소속 상세정보
이대일/Lee Dale
KMID : 0360319790110010031
Abstract
Pinellia tumberg agglutinin was purified from saline extract by ammonium sulfate (60%) precipitation and successive DEAE Sepharose chromatography in 0.05M Tris HCL pH 8.0 with linear NaCl gradient. An active fraction was eluted in 0.1~0.25M NaCl and its agglutinating activity to L5178Y was 2,500 units/mg with high cytotoxicity to L5178Y in saline suspension but weak toxicity in Fischer´s medium.
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원문 및 링크아웃 정보
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