인체신경아세포종세포주 SK-N-SH, IMR-32, SK-N-MC에 대한 Cytokine의 항암효과에 관한 연구
The Antiumor Effect of Various Cytokines on Human Neuroblastoma Cell Lines SK-N-SH, IMR-32, and SK-N-MC
황태숙, 송형근, 박성희, 함의근,
소속 상세정보
황태숙 ( )
인하대학교
송형근 ( )
인하대학교
박성희 ( )
인하대학교
함의근 ( )
인하대학교
KMID : 0360319920240010035
Abstract
Cytokine, a messenger of the immune system may inhibit cell growth and proliferation and enhance cytolytic activity by modulating immune responses. Extensive studies using 12 cytokines(human recombinant IFN-a, IFN-r, IL-1a, IL-1β, IL-2, IL-3,
IL-4,
IL-6, GM-CSF, M-CSF TNF-a, and TNF-β) wee performed in order to investigate the antiproliferative and cytolytic activity of the various cytokines on human neuroblastoma cell lines SK-N-SH, IMR-32 and SK-N-MC.
The antiproliferative activities were assayed by 쿓-thymidine uptake test and the cytolytic activities by 51Cr release assay. Among the 12 cytokines, IFN-a, IFN-r, and IL-2 revealed remarkable antiproliferative and cytolytic activity and these
activities varied among different cell lines.
The IFN-r treated natural killer cells did not show any significant increase in cytolytic activity as compared to that of the untreated natural killer cells, and the same results were obtained with monocytes. This cytolytic activity was
significantly
increased when natural killer cells were co-culture with supernate of IFN-r-treated monocytes.
The same result was obtained with IFN-a except that the IFN-a-treated natural killer cells also showed a relatively significant increase in cytolytic activity.
As was previously reported, IL-2 enhance cytolytic activity by stimulating natural killer cells.
Based on the above results, it seems reasonable to say that the IFN-r, IFN-a, and IL-2 have a considerable immunologic effect on various subtypes of peripheral mononuclear cells, and the cytolytic activity of IFN-r and IFN-a on human
neuroblastoma
cell
line is mediated mostly through monocyte-natural killer cell pathway, although it possible that IFN-a could also stimulate natural killer cell activity in a direct way.
키워드
원문 및 링크아웃 정보
등재저널 정보