악성 위암 환자에서 항암제가 호중구의 탐식작용에 미치는 영향 - Flow cytometry의 응용
The Effect of Chemotherapeutic Agents on Phagocytosis of Polymorphonuclear Leukocytes in Patients with Gastric Cancer -Application of flow Cytometry-
대한암학회지 1992년 24권 2호 p.249 ~ 255
소속 상세정보
원종호
라동집/우준희/홍대식/박희숙/이희발
KMID : 0360319920240020249
Abstract
FAM(5-fluorouracil, adriamycin, mitomycin-C) combination chemotheapy regimen is widely used in stomach cancer patients. The in vitro effect of therapeutic concentrations of FAM on the phagocytosis of Staphylococcus aureus by human
polymorphonuclear
leukocytes(PMNL) was assessed with flow cytometry. PMNL were isolated from whole blood of eleven healthy controls and eleven patients with untreated gastric cancer by density gradient centrifugation, Cells were incubated with 5-fluouracil
51.8ug/ml,
adriamycin 2.6킽/ml, and mitomycin-C 0.87킽/ml. The phagocytic activity of PMNL was compared at times 0, 1, and 4 hours after incubation.
1) The phagocytic activity was significantly lower in patients with gastric cancer compared with controls at baseline(p<0.05).
2) The phagocytic activity decreased in both controls and patients at 1 hours of incubation (p<0.05). The phagocytic activity at 1 hours was significantly lower in patients than in controls.
3) The phagocytic activity showed tendency to increase at 4 hours as compared to that of 1 hours in both controls and patients.
In conclusion, the baseline phagocytic activity was significantly lower in patients with gastric cancer and FAM reduces phagocytic activity of PMNL at 1 hours in both controls and patients. The phagocytic activity was significantly lower in the
patients
than in controls at 1 and 4hours after treatments with FAM
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