3주령 흰쥐에서 디에칠니트로사민 투여에 의한 GST-P+ foci의 유도: GST-P+ foci를 이용한 새로운 중기 간 발암 실험법의 시도
Development of GST-P+ Foci in 3 Week Old Rats Induced by Ditthylnitrosamine: Preliminary data for liver foci model
대한암학회지 1994년 26권 2호 p.231 ~ 235
소속 상세정보
장자준
이윤실/이미숙/김태환
KMID : 0360319940260020231
Abstract
A new medium-term in vivo hepatocarcinogenesis model was tried using GST-P+ hepatic foci. The treatment protocol was the i. p. injection of 50mg/kg body weight of diethylnitrosamine(DEN) once, twice or three times per week with or without 0.05%
phenobarbital(PB) in 3 week female Sprague-Dawley rats or male Fischer 344 rats. After 9 weeks, the rats were sacrificed and their livers were examined for immunohistochemically detectable GST-P+ foci. DEN treatment in 3 week old rats without PB
was
enough to induce GST-P+ foci. Protocol is very simple and convenient compared to other liver foci model using newborn rats or combined partial hepatectomy. And the yield of GST-P+ foci treated with DEN and PB was almost equal as other models.
Therefore,
this simple hepatic foci model may be useful for screening of carcinogenic or anticarcinogenic agents. For the validity of new model, more extensive studies will be necessary.
키워드
원문 및 링크아웃 정보
등재저널 정보