후두 편평상피의 전암성 및 악성병변에서 화상분석기를 이용한 핵산 검사와 Ki-67항체 양성세포의 분석
DNA Content and Ki-67 Antibody Expression by Means of Image Analyszer for Laryngeal Lesions
대한암학회지 1994년 26권 3호 p.466 ~ 473
최종구, 이선희, 김주현, 김인선,
소속 상세정보
최종구 ( )
고려대학교
이선희 ( )
고려대학교
김주현 ( )
고려대학교
김인선 ( )
고려대학교
KMID : 0360319940260030466
Abstract
The laryngeal epithelial cell kinetics of 26 laryngeal lesions(invasive squamous cell carcinoma 14, epithelial hyperplasia 5, laryngeal nodule 7) were studied by immunohistochemical analysis with the monoclonal antibody Ki-67, which reacts with a
nuclear antigen in proliferating cells using paraffin embedded tissue. For DNA analysis, touch imprint with fresh biopsy specimens were stained with Feulgen and analyzed by image analyzer in 22 cases.
1) The positive nuclear area of Ki-67 were 32.65±11.59% in invasive squamous cell carcinoma, 2014(3.38% in epithelial hyperplasia and 11.66±3.02% in laryngeal nodule.
2) DNA aneuploidy was found in 7 cases of 10(70%) invasive squamous cell carcinomas, 2cases of 5(40%) epithelial hyperplasia and none of laryngeal nodules.
3) Average proliferative index(S phasa+G2/M phases) was 24.32±11.33% in spuamous cell carcinoma, 13.09(10.90% in epithelial hyperplasia and 4.50±1.19% in laryngeal nodule.
As the results, the measurement of the DNA content and Ki-67 positive nuclear area on the small biopsy obtained by microscopic surgery used as effective factors predicting prognosis.
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