유방암에서 유세포분석의 의의
The Significance of DNA Flow-cytometry in Breast Cancer
대한암학회지 1997년 29권 5호 p.738 ~ 747
백인경, 정우희, 이희대,
소속 상세정보
백인경 ( )
연세대학교 의과대학 외과학교실
정우희 ( )
연세대학교 의과대학 병리학교실
이희대 ( )
연세대학교 의과대학 외과학교실
KMID : 0360319970290050738
Abstract
결론
유방암에서 DNA flowcytometry 분석상 diploidy (34.5%)보다는 aneuploidy(65.5%)가 많
았으며 S-phase fraction의 중앙값은 11.2%이었고 aneuploidy의 경우 diploidy에 비하여 높
은 SPF 수치를 보였다(p<0.05). 또한 림프절 전이가 있는 유방암의 경우 전이가 없는 경우
에 비하여 높은 수치의 SPF수치를 보여(p<0.05) DNA 함량의 변화가 기존의 예후인자들과
연관성을 가지면서 측정가능한 예후인자로서 가능성이 있음을 알 수 있었다. 예후적 가치를
위한 연구에서 DNA ploidy나 S-phase fraction에 따른 생존율 및 무병생존율은 aneuploidy
이거나 high SPF의 경우가 diploidy나 low SPF에 비하여 통계적 유의성은 없었지만 높은
수치를 보여 좀더 장기간의 추적조사를 통하여 예후인자 및 예측인자로서의 역할을 알 수
있을 것으로 기대된다.
#초록#
Purpose : To evaluate the relationship between nuclear DNA contents and prognostic
factors and survival in breast cancer patients.
Materials and Methods : We determined nuclear DNA content from 91
paraffin-embedded malignant breast tumors and evaluated relationship between DNA
nuclear content and well-known prognostic indicators of breast cancer and the survival
of the patients by statistical analyses.
Results : Twenty nine(34.5%) of the 91 tumors examined were diploid, and the
remainder(65.5%) contained one or more aneuploid clones. 5-phase fraction(SPF) ranged
from 1.4 to 68.3%(median 11.2%) and it was higher in aneuploidy tumors than in diploid
tumors(p<0.05). Positive axillary lymph nodes were found in 72.7% of the patients who
had a tumor with a high SPF (above the median 11.2%) and in 27.3% of those with
tumor with low SPF (below median)(p<0.05). The overall survival rate was 96.1% in
DNA diploid and 87.6% in DNA aneuploid tumors, showing that DNA ploidy had no
prognostic significance in breast cancers. The actuarial survival rates were 96.4% and
86.3% for low and high SPF, respectively(p=0.28). The patients with high SPF showed
high disease free survival rate compared to the patients with low SPF but the difference
had no statistical significance.
Conclusion : Our results indicate DNA aneuploid tumors were more prevalent in
breast cancer patients with high SPF or lymph node metastasis and larger patient
accumulation with longer follow-up period will be helpful to identify the relationship
between flow-cytometrical analysis and prognosis.
키워드
DNA flow-cytometry; S-phase fraction; DNA index; Breast cancer;
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