한국인의 비호지킨 림프종에서 Epstein-Barr Virus 검출의 의의
Significance of Epstein-Barr Virus Detection in non-Hodgkin
대한암학회지 1997년 29권 5호 p.851 ~ 866
소속 상세정보
서창인/Chang In Suh
김범준/박재원/황응수/국윤호/차창룡/Bum-Joon Kim/Jae-Won Park/Eung-Soo Hwang/Yoon-Hoh Kook/Chang-Yong Cha
KMID : 0360319970290050851
Abstract
결론
우리나라 비호지킨 림프종 환자의 28.8%에서 조직내 EBV가 검출되며 특히 polymorphic
reticulosis를 포함한 T세포 림프종에서 검출률이 높았다. Southers blot hybridization 상
EBV는 비호지킨 림프종 내에 단클론성으로 잠복 감염되어 있어, EBV는 림프종 발병후의
기회감염이라기보다는 림프종 형성이전부터 존재하여 림프종 발병과정에 기여했을 것으로
추정된다. EBV의 림프종 형성과정에서의 구체적인 역할과 예후에 미치는 영향은 앞으로 연구되어야 할 과제이다.
#초록#
Purpose : To investigate whether non-Hodgkin's lymphoma of Korea is pathogenetically associated with Epstein-Barr virus(EBV).
Materials and Methods : We analyzed fifty nine paraffin-embedded tissue and 22 fresh
frozen tissue samples from non-Hodgkin's lymphoma patients for the presence of EBV
sequences by polymerase chain reactions(PCR), in situ hybridization(ISH) and assessed
the clonality of EBV infected cells by Southern blot hybridization.
Result : On ISH using oligonucleotide probes corresponding to EBV-encoded small
RNAs (EBERs), 17(28.8%) of 59 paraffin-embedded tissue samples showed positive
hybridization signals localized over the nuclei of the tumor cells, but PCR using primers
from Internal Repeat Ⅰ or EBV-determined nuclear antigen 1 gene showed positive
results in only 6(10.2%) and 5(8.5%) samples, respectively. ISH and PCR did not detect
EBV sequences in 15 paraffin-embedded tissue samples of tuberculous lymphadenitis
patients. In 22 fresh frozen tissue samples, PCR detected EBV sequences in three
samples from peripheral T cell lymphoma(PTCL). In two of those three samples,
Southern blot analysis showed that these viral DNAs were monoclonal and of latent
form.
Conclusion : Approximately 28.8% of non-Hodgkin's lymphoma were related to EBV
in Korea. Monoclonality of those EBV DNAs implies that virus infection preceded
malignant transformation, suggesting that EBV may play a role in lymphomagenesis.
키워드
Epstein-Barr virus; Non-Hodgkin's lymphoma; Monoclonality; Lymphomagenesis;
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