Epstein-Barr Virus의 향성에 관한 연구
A Study on the Tropism of Epstein-Barr Virus
대한암학회지 1997년 29권 6호 p.954 ~ 964
정춘해, 이미자, 홍순표,
소속 상세정보
정춘해 ( )
조선대학교 의과대학 내과학교실
이미자 ( )
조선대학교 의과대학 병리학교실
홍순표 ( )
조선대학교 의과대학 병리학교실
KMID : 0360319970290060954
Abstract
결론
CD2l 항원은 CD20 또는 CDl9에 양성인 B 세포, 일부 CD5 양성 B 림프구, 일부 EBV-양
성 또는 음성 B세포 림프종 세포주, 조직구성 림프종 세포주에서 발현되었으며 T 림프구나
T 세포 림프종 및 백혈병 세포주 그리고 위암 세포주에서는 발현되지 않았다.
따라서 EBV는 CD2l 항원을 발현하고 있는 B세포 또는 악성 림프종 B 세포주에 향성을
지니고 있으며 CD5항원과 CD2l 항원을 동시에 발현하는 멘틀세포에 대한 EBV의 향성에
대해서는 보다 더 많은 연구가 필요하다고 생각된다.
#초록#
Purpose : The Epstein-Barr Virus(EBV) is associated with a variety of human
lymphocytic and epithelial malignancies. EBV is thought to display exclusive tropism for
B loymphocytes, follicular dendritic cells, and pharyngeal epithelia via specific receptors
(C3d receptors, CR2, CD21). Recent evidence, however, challenged this belief. We
designed this experiment to determine the incidence of EBV receptor in various
malignant tumor cell lines and normal lymphocyte subsets.
Materials and Methods : We have examined the incidence of EBV receptor, CD21 on
the 10 healthy adult peripheral blood(PB), 10 umbilical cord blood(CB), 4 immortalized
lymphoblastoid B cells by EBV infection(CSUP-1, CSUP-2, CSUP-3, CSUP-4), 3
EBV-positive B cell lymphoma cell lines(Jiyoye, IM-9, PTLC-1), 1 EBV-negative B cell
lymphoma cell line(JeKo-1), 3 T cell lymphoma and leukemia cell lines(CCRF-CEM, H9,
CEM-CM3), one histiocytic lymphoma cell line(U-937) and 5 gastric cancer cell lines
(KATO Ⅲ, AGS, SNU-1, SNU-5, and SNU-16). EBV receptor, C3d receptor was
identified by flow cytometry(FACSCalibur) using FITC-conjugated or PE-conjugated
CD21 monoclonal antibody. Also we investigated the expression of CD3, CD5, CD7, CD
19, CD2O, IgM, IgG, IgK and IgL by using FITC-conjugated or PE-conjugated
monoclonal antibody, on above cell lines.
Results : The expressions of CD2l molecule were 10.99± 3.84% and 9.22 ±5.39% in
adult PB lymphocytes and CB lymphocytes, respectively. The anti-human CD21 antibody
was positive for CD 19-positive or CD2O-positive B lymphocytes. The CD3-positive or
CD7-positive T lymphocytes were negative for anti-human CD2l antibody in PB and
CB. But, CD21 antibody was weakly positive for CD5-positive Lymphocytes.
EBV-positive cell lines expressed variable ranges from 0.9% to 5.2% for CD2l antigen,
while EBV-negative lymphoma cell line, JeKo-1 expressed 5.5%. All T lymphoma and
leukemia cell lines and gastric cancer cell lines did not express CD21 antigen. But
U-937 expressed 14.4% for CD2l antigen.
Conclusion : These results suggested that the CD2l antigen was expressed in CD2O
or CDl9-positive mature B cells, CD5-dim positive lymphocytes, some EBV-positive and
negative B cell lymphoma cell lines, and a histiocytic lymphoma cell line. Further
evaluation on the nature of CD5-dim positive cells, which was expressing CD21
molecule, is revealed, especially in reference to EBV association in some peculiar
subtypes of peripheral T cell lymphoma.
키워드
Epstein-Barr virus; Epstein-Barr virus receptor; CD2l; Flow cytometry; Cell line;
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