폐의 비소세포암에서 p53유전자 돌연변이와 TGF-β에 관한 분자생물학적 연구
TGF-beta-1 Expression and p53 Mutation in Non-small Cell Carcinomas of the Lung
대한암학회지 1997년 29권 6호 p.1022 ~ 1031
소속 상세정보
김한겸/Han Kyeom Kim
박설희/나영순/강용구/김영식/한정호/박미자/김인선/Seol Hee Park/Young Soon Na/Yong Gu Kang/Young Sik Kim/Jung Ho Han/Mee Ja Park/Insun Kim
KMID : 0360319970290061022
Abstract
결론
폐암중 비소세포암에서는 p53유전자 돌연변이가 46% 정도의 빈도로 관찰되었고 TGF-β
-1 mRNA발현율이 높았는데 이는 953 유전자 돌연변이에 의해 형질 전환된 암세포가
TGF-β-1에 대한 세포 증식 억제 효과로부터는 벗어나지만 한편으로는 TGF-β-1이 종양
간질의 축척 및 혈관 신생을 유도하므로 결국은 p53유전자 돌연변이와 TCF-β-1이 종양의
발생과 증식을 유발하는데 기여한다고 생각된다. 그러나 이러한 가설을 증명하고, p53과
TGF-β-1의 상호 관계뿐 만 아니라 폐암의 발생과 진행과정의 어느 단계에 관여하는 지를
명확하게 알기 위해서는 더 많은 연구가 이루어져야 하겠다
#초록#
Purpose : TGF-beta-1 is actually a major growth inhibitor for most cell types. We
assumed that the loss of TGF-beta-1 would be occurred during carcinogenesis of the
lung. Also, the mutation and expression of p53 have been known to be major molecular
change of non-small cell carcinoma of the lung. So, the relationship between the
mutation of p53 and the expression of TGF-beta-1 in the non-small cell carcinomas
were evaluated.
Materials and Methods : In 43 non-small cell carcinoma and normal tissue of the
lung, their TGF-beta-1 mRNA were measured by RT-PCR and p53 was studied by
SSCP and Western blotting assay.
Results : p53 mutation rate in non-small cell carcinomas of the lung (48.4%) was
much more frequent than the normal control group (14.3%). The expression rate of
TGF-beta-1 in lung carcinomas, especially squamous cell carcinoma (71.4%), was much
higher than the normal control group (42.9%). p53 mutation and TGF-beta-1 mRNA in
the lung carcinomas were not strongly correlated.
Conclusion : It suggests that high expression rate of TGF-beta-1 and p53 mutation
are associated with carcinogenesis of non-small cell carcinoma of the lung. High
expression rate of TGF-beta-1 in the lung carcinomas can be partly explained by the
fact that TGF-beta-1 have capacity to control the production of many components of
the extracellular matrix and enhance angiogenesis in favor of tumor growth despite of
their inhibitory effects of cell growth. However, additional research is required to
determine the exact role of TGF-beta-1 in carcinogenesis of the lung.
키워드
TGF-beta-1; p53; Non-small cell carcinoma;
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