한국인 위암 세포주에서 세포주기 조절 유전자의 발현
Expression of Cell Cycle Control Genes in Korean Gastric Cancer Cell Lines
대한암학회지 2000년 32권 2호 p.279 ~ 287
임춘철, 김인호, 조재위, 백원기, 서성일, 서민호, 박종욱, 강중신, 손수상,
소속 상세정보
임춘철 ( Lim Chun-Cheol )
계명대학교 의과대학 외과학교실
김인호 ( Kim In-Ho )
계명대학교 의과대학 외과학교실
조재위 ( Cho Jae-We )
서남대학교 의과대학 미생물학교실
백원기 ( Baek Won-Ki )
계명대학교 의과대학 미생물학교실
서성일 ( Suh Seong-Il )
계명대학교 의과대학 미생물학교실
서민호 ( Suh Min-Ho )
계명대학교 의과대학 미생물학교실
박종욱 ( Park Jong-Wook )
계명대학교 의과대학 면역학교실
강중신 ( Kang Joong-Shin )
계명대학교 의과대학 외과학교실
손수상 ( Sohn Soo-Sang )
계명대학교 의과대학 외과학교실
KMID : 0360320000320020279
Abstract
Purpose : In order to elucidate the roles of the cell cycle control genes in the carcinogenesis of Korean gastric cancer, the expressions of cyclin A, D1, E, p16INK4a and Rb in Korean gastric cancer cells were investigated in the study.
Materials and Methods : The expression patterns of major cell cycle control genes in Korean gastric cancer cells (SNU1, 5, 216, 484, 601, 620, 638, 668, 710) were analysed by using western blot and reverse transcription-polymerase chain reaction (RT-PCR).
Results : In most of the gastric cancer cells investigated, the overexpression of cyclin A and hyperphorylated Rb, and markedly decreased expression of p16INK4a were observed. Over-expression of cyclin E was also observed in SNU1, 484, 601 and 638 gastric cancer cells.
Conclusions : Considering all these findings, the following model of the gastric carcinogenesis caused by the altered expression of the cell cycle control genes can be postulated; over-expressions of cyclin A and E cause acceleration of Rb phosphorylation, inactivation of Rb functions and acceleration of G1/S transition of the cells; decreased expression of p16INK4a causes inadequate inhibition of cyclin-CDK and further acceleration of Rb phosphorylation; consequently, Rb inactivation, G1 shortening, inappropriate cell division and decreased function of the maintenance of genomic stability occurs. All these abnormalities should contribute to the carcinogenesis of Korean
gastric cancer cells.
키워드
Stomach neoplasm;Cell cycle control genes;
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