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Effect of Hepatocyte Growth Factor on the Expression of E-cadherin in Gastric Carcinoma Cell Lines
ÇÑ»ó¿í, ÀÌ¿øÈï, ±è¿íȯ, ±è¸í¿í, ÀÌÀçÈ£, ¼Û»ó¿ë, ÀÌ°Ç¿í,
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ÇÑ»ó¿í ( Han Sang-Uk )
¾ÆÁÖ´ëÇб³ ÀÇ°ú´ëÇÐ ¿Ü°úÇб³½Ç
ÀÌ¿øÈï ( Lee Won-Hung )
¾ÆÁÖ´ëÇб³ ÀÇ°ú´ëÇÐ ¿Ü°úÇб³½Ç
±è¿íȯ ( Kim Wook-Whan )
¾ÆÁÖ´ëÇб³ ÀÇ°ú´ëÇÐ ¿Ü°úÇб³½Ç
±è¸í¿í ( Kim Myung-Wook )
¾ÆÁÖ´ëÇб³ ÀÇ°ú´ëÇÐ ¿Ü°úÇб³½Ç
ÀÌÀçÈ£ ( Lee Jae-Ho )
¾ÆÁÖ´ëÇб³ ÀÇ°ú´ëÇÐ »ýÈÇб³½Ç
¼Û»ó¿ë ( Song Sang-Yong )
¼º±Õ°ü´ëÇб³ ÀÇ°ú´ëÇÐ »ï¼º¼¿ïº´¿ø Áø´Üº´¸®°ú
ÀÌ°Ç¿í ( Lee Kuhn-Uk )
¼¿ï´ëÇб³ ÀÇ°ú´ëÇÐ ¿Ü°úÇб³½Ç
KMID : 0360320000320050852
Abstract
Purpose :Previously, we reported that the expression of E-cadherin was significantly decreased according to the increase of the level of hepatocyte growth factor (HGF) in gastric cancer tissue. In this work, the effect of HGF on the cell-cell adhesion and intracellular distribution of E-cadherin in the gastric carcinoma cell lines were studied.
Materials and Methods :Western blot analysis was performed to confirm the presence or abscence of c-Met and E-cadherin in SNU-1, 5, and 16 cells. Tyrosine phosphorylation of c-Met, E-cadherin, ¥á-, ¥â-, ¥ã-catenins was checked by immunoprecipitation. The morphologic changes induced by HGF were studied with immunocytochemical staining. Functional proportion of E-cadherin was estimated by cell fractionation. The effect of HGF on cell proliferation and invasion was also assessed.
Results :Among SNU-1, 5, and 16 cell lines, only SNU-16 cells expressed both E-cadherin and c-Met. A morphological change from epithelial shape to fibroblastic one was observed in the SNU-16 cells after treatment with HGF. In addition, E-cadherin expression of the SNU-16 cells was shifted from the membrane and to the cytoplasm, and the functional fraction of E-cadherin was decreased in the SNU-16 cells treated with HGF. On the other hand, HGF increased the proliferation and invasion of the SNU-16 cells.
Conclusion :These results suggest that HGF may regulate cell adhesion in gastric carcinomas via the cellular redistribution and functional change of E-cadherin.
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Stomach neoplasm;Hepatocyte growth factor;c-Met gene;E-cadherin;Invasion;
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