Clinical Significance of Peripheral Blood CEA mRNA Expression in Gastric Cancer patients Underwent Curative Resection
대한암학회지 2001년 33권 6호 p.483 ~ 488
서재홍, 박선희, 백창원, 최철원, 김병수, 신상원, 김열홍, 김준석, 목영재, 김종석, 한선애, 윤정인,
소속 상세정보
서재홍 ( Seo Jae-Hong )
고려대학교 의과대학 내과학교실
박선희 ( Park Sun-Hee )
고려대학교 의과대학 내과학교실
백창원 ( Bak Chang-Won )
고려대학교 의과대학 내과학교실
최철원 ( Choi Chul-Won )
고려대학교 의과대학 내과학교실
김병수 ( Kim Byung-Soo )
고려대학교 의과대학 내과학교실
신상원 ( Shin Sang-Won )
고려대학교 의과대학 내과학교실
김열홍 ( Kim Yeul-Hong )
고려대학교 의과대학 내과학교실
김준석 ( Kim Jun-Suk )
고려대학교 의과대학 내과학교실
목영재 ( Mok Young-Jae )
고려대학교 의과대학 외과학교실
김종석 ( Kim Chong-Suk )
고려대학교 의과대학 외과학교실
한선애 ( Han Sun-Ae )
고려대학교 의과대학 병리학교실
윤정인 ( Yoon Jung-In )
고려대학교 Graduate School of Biotechnology
KMID : 0360320010330060483
Abstract
Purpose: Recent advances in molecular technology have made it possible to detect small numbers of circulating tumor cells in the peripheral blood or bone marrow. Carcinoembryonic antigen (CEA) is an oncofetal antigen that is expressed in epithelial tumor cells. CEA mRNA may be a reliable marker for the detection of tumor cells in the peripheral blood of patients with epithelial cancer.
Materials and Methods: We analyzed the peripheral blood of 46 patients with gastric cancer who had un-dergone curative resection. The presence of CEA mRNA was serially monitored using RT-PCR (Preop, Postop 15 day, 2 months (m), 4 m, 6 m, 8 m, 10 m, 12m). The clinical characteristics, serum CEA level and immunohisto-chemical staining of tumor tissue were also evaluated. Patients were followed up for 6 to 12 months.
Results: There was no significant relationship seen between CEA mRNA RT-PCR positivity in the peripheral blood and sex, stage, serum CEA level or immunohisto-chemical staining for CEA antigen, During follow up, eight patients experienced recurrence ; were positve for CEA mRNA RT-PCR recurrence was seen in 66.7% (6/9) of the patients who before clinical recurrence as com-pared to 5.4% (2/37) of patients who were negative (p=0.0002). Serial changes of CEA mRNA RT-PCR corr-elated with clinical recurrence ; 100% in the positively converted group (3/3), 0% in the negatively converted group(0/18), 50% in all positive group (3/6) and 10.5% in all negative group (2/ 19) experienced recurrence, respec-tively.
Conclusion: RT-PCR analysis of CEA mRNA in the peripheral blood seems to be a promising tool for the early detection of micrometastatic circulating tumor cells in gastric cancer patients and may be useful in determining patients at high risk for recurrence. However, definitive correlation with recurrence certainly requires a longer follow up duration in furthers tudies.
키워드
Stomach neoplasm;CEA mRNA;
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