Nested 중합효소 연쇄반응법을 이용한 화상 환자에서의 녹농균 검출 -혈액 배양법과의 비교-
Identification of Pseudomonas Aeruginosa Isolated from Burn Patients by Nested Polymerase Chain Reaction -Special reference to comparison with conventional blood culture-
임민균, 김동건, 김정진, 김종현, 김이수, 우영민, 김성, 윤대원, 최창식,
소속 상세정보
임민균 ( Im Min-Gyun )
한림대학교 의과대학 한강성심병원 외과학교실
김동건 ( Kim Dong-Kun )
한림대학교 의과대학 한강성심병원 외과학교실
김정진 ( Kim Jung-Jin )
한림대학교 의과대학 한강성심병원 외과학교실
김종현 ( Kim Jong-Hyun )
한림대학교 의과대학 한강성심병원 외과학교실
김이수 ( Kim Lee-Su )
한림대학교 의과대학 한강성심병원 외과학교실
우영민 ( Woo Young-Min )
한림대학교 의과대학 한강성심병원 외과학교실
김성 ( Kim Sung )
한림대학교 의과대학 한강성심병원 외과학교실
윤대원 ( Yoon Dal-Won )
한림대학교 의과대학 한강성심병원 외과학교실
최창식 ( Choi Chang-Sig )
한림대학교 의과대학 한강성심병원 외과학교실
KMID : 0371320010600010016
Abstract
Purpose: Pseudomonas aeruginosa has emerged as one of the most problematic bacteria in modern hospital settings, and this organism is increasingly isolated as a nosocomial pathogen. Burn patients are particularly susceptible to Pseudomonas infection. Therefore, the accurate and sensitive microbiologic tests are needed for strict management of this prevalent microorganism.
Methods: A nested polymerase chain reaction test based on consecutive amplification of the lipoprotein genes, oprL and oprI, was designed and evaluated, in comparison with the conventional blood culture, for its ability to detect Pseudomonas aeruginosa in clinical materials of burn patients.
Results: Positive results of PCR based on oprL gene were observed only for Pseudomonas aeruginosa. All other bacteria (n=4) tested by this amplification method were negative. Also the lowest detection level was 1×101 bacteria per ml of blood samples. In addition, PCR afforded a significantly higher detection rate for Pseudomonas aeruginosa than the conventional blood culture technique in clinical materials of burn patients (25.9% vs. 8.6%).
Conclusion: This study suggests that the nested PCR technique is highly specific and sensitive test for detection of Pseudomonas aeruginosa, and therefore it may be a useful adjunct tool, in combination with other conventional techniques, for detection of Pseudomonas aeruginosa infection.
키워드
녹농균;중합효소 연쇄반응법
Pseudomonas aeruginosa;Polymerase chain Reaction;
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