유방암에서 액와 림프절 미세전이의 진단을 위한 역전사-중합효소 연쇄반응의 의의 MUC1, Cytokeratin 19의 결과 비교
Detection of Axillary Lymph Node Micrometastases in Breast Cancer Using RT-PCR Comparison the Results of MUC1, Cytokeratin 19
이령아, 오승근, 최국진, 윤여규, 노동영, 김성원, 강희준, 강한성, 김성숙,
소속 상세정보
이령아 ( Lee Ryung-Ah )
서울대학교 의과대학 외과학교실
오승근 ( Oh Seung-Keun )
서울대학교 의과대학 외과학교실
최국진 ( Choi Kook-Jin )
서울대학교 의과대학 외과학교실
윤여규 ( Youn Yeo-Kyu )
서울대학교 의과대학 외과학교실
노동영 ( Roh Dong-Young )
서울대학교 의과대학 외과학교실
김성원 ( Kim Sung-Won )
서울대학교 의과대학 외과학교실
강희준 ( Kang Hee-Joon )
서울대학교 의과대학 외과학교실
강한성 ( Kang Han-Sung )
시립보라매병원 일반외과
김성숙 ( Kim Seong-Suk )
울산대학교 의과대학 병리학교실
KMID : 0371320010600010029
Abstract
Purpose: The detection of axillary lymph-node micrometastases in breast-cancer patients by using the reverse transcriptase-polymerase chain reaction (RT-PCR) may provide a good guide for postoperative therapy. To evaluate effectiveness of RT-PCR for the detection of micrometastasis, we have compared the results of conventional, immunohistochemical (IHC) staining with those of RT-PCR.
Methods: We conducted RT-PCR amplifications of MUC1 and cytokeratin (CK) 19 on lymph nodes from 40 breast- cancer patients and conducted IHC staining for cytokeratin. The results of histological examination and cytokeratin IHC staining were compared with the RT-PCR results for the detection of lymph-node micrometastases.
Results: Nineteen of 40 cases (47.5%) were lymph-node positive and twenty-one cases (52.5%) were lymph-node negative. Using RT-PCR MUC1 and CK19 expressions were detected in all positive lymph nodes and in 4 (19.0%) and 5 (23.8%), in negative nodes, respectively. Not all positive nodes were stained by IHC (94.7%), and none of the negative lymph nodes were stained by IHC. Thus, IHC staining was ineffective in detecting micrometastases.
Conclusion: Detection of MUC1 and CK19 by using RT- PCR can be a more accurate and useful method than IHC staining for the detection of axillary lymph-node micro-metastases in breast cancer.
키워드
유방암;림프절 미세전이;역전사-중합효소연쇄반응
Breast cancer;Lymph node micrometastases; MUC1;Cytokeratin 19; RT-PCR
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