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Tissue Engineering of Vascular Prosthetic Grafts Using Autogenous Vein Cells
김동익, 이병붕, 정현아, 정연주, 김지은, 한진수, 박정환, 허세호, 서수원,
소속 상세정보
김동익 ( Kim Dong-Ik )
성균관대학교 의과대학 삼성서울병원 혈관외과
이병붕 ( Lee Byung-Boong )
성균관대학교 의과대학 삼성서울병원 혈관외과
정현아 ( Jeong Hyeon-A )
성균관대학교 의과대학 삼성서울병원 혈관외과
정연주 ( Jung Yeon-Joo )
성균관대학교 의과대학 삼성서울병원 삼성생명과학연구소
김지은 ( Kim Ji-Eun )
성균관대학교 의과대학 삼성서울병원 삼성생명과학연구소
한진수 ( Han Jin-Soo )
성균관대학교 의과대학 삼성서울병원 삼성생명과학연구소
박정환 ( Park Jung-Hwan )
성균관대학교 의과대학 삼성서울병원 삼성생명과학연구소
허세호 ( Huh Se-Ho )
성균관대학교 의과대학 삼성서울병원 혈관외과
서수원 ( Suh Soo-Won )
성균관대학교 의과대학 삼성서울병원 의공학과
KMID : 0371320010600060579
Abstract
Purpose: To investigate bioartificial vessels capable of being used for vascular grafts, we studied cell-polymer constructs from venous smooth muscle cells (SMCs) and biodegradable scaffolds using the canine model.
Methods: Scaffolds constructed from 50/50 poly (D,L- Lactide-co-glycolide)(PLGA) were created with pores containing gelatin particles. Disk type scaffolds were used as templates of cell attachment and vascular tissue regeneration. SMCs were isolated from canine external jugular veins and primary SMCs cultures were produced with the explant- derived method. SMCs were seeded into the scaffolds and cultured statically for 4 weeks. The cell-polymer constructs were examined histochemically and using scanning electron microscopy.
Results: The SMCs obtained by the explant-derived method were confirmed with immunohistochemical staining using an anti-smooth muscle actin antibody. Four weeks after the SMCs were seeded into the scaffold, histological examination showed SMCs infiltration into the scaffold wall and scanning electron microscopy revealed the SMCs mass which resembled tissue on the scaffold surface.
Conclusion: This is a pilot study for the constructing artificial vessels using tissue engineering. The construction of the ideal scaffold for vessel and the improvement of culture methods in vitro are the most important parts in this field.
키워드
조직공학;이식편;지지체
Tissue engineering;Graft;Scaffold
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