유방암조직의 Tissue Microarrays에서의 면역화학염색법을 이용한 Apoptosis 관련단백질의 발현
Apoptosis Related Protein Expressions in Immunohistochemical Staining Using Tissue Microarrays of Breast Cancer
김정수, 김기환, 안창혁, 전해명, 정상설, 임석아,
소속 상세정보
김정수 ( Kim Jeong-Soo )
가톨릭대학교 의과대학 의정부성모병원 외과
김기환 ( Kim Kee-Hwan )
가톨릭대학교 의과대학 의정부성모병원 외과학교실
안창혁 ( An Chang-Hyeok )
가톨릭대학교 의과대학 의정부성모병원 외과학교실
전해명 ( Jeon Hae-Myung )
가톨릭대학교 의과대학 의정부성모병원 외과학교실
정상설 ( Jung Sang-Seol )
가톨릭대학교 의과대학 의정부성모병원 외과학교실
임석아 ( Im Seock-Ah )
이화여자대학교 의과대학 내과학교실
KMID : 0371320010600060606
Abstract
Purpose
In order to confirm the clinical application of a tissue microarrays method, the expression rate and relationship between factors related apoptosis, hormonal receptors and the clinical factors were investigated.
Methods
A tissue microarrays of 59 breast cancer tissues, and apoptosis related factors were examined by immunohistochemical staining using monoclonal antibodies.
Results
The median age of the patients was 49.9 years and 86.4% had a pathological stage of over stage II. The average number of metastatic lymph nodes was 3.8. p53 expression was noted in 21 cases (35.6%) and was related to Bcl-2, ER and PR expression.
PTEN
was expressed in 39 cases (66.1%) and related to FAS, Bcl-2, ER and PR expression. Fas was expressed in 34 cases (57.6%) and related with PR and BAX expression. BAX expression was observed in 42 cases (71.2%) and was related to the metastatic
axillary
lymph nodes, and both Bcl-2 and PR expression. Bcl-2 expression was noted in 33 cases (55.9%) and related to ER and PR expression. ER was expressed in 34 cases (57.6%) and was related positively with PR expression.
Conclusion
The tissue microarrays method can be used for both screening and analyzing many factors or different tumor types. This new technique may be very powerful for the rapid identification of the tumor characteristics.
키워드
조직미세배열; 고사; 유방암; Tissue microarrays; Apoptosis; Breast cancer;
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