백서의 일차배양 간세포에서 Plasminogen Activator와 Plasminogen Activator Inhibitor의 표피 성장인자 의존적 발현에 관한 연구
Epidermal Growth Factor Dependent Expression of Plasminogen Activator and Plasminogen Activator Inhibitor in Rat Hepatocytes in Primary Culture
윤완희, 최정훈, 정연주, 이병학, 김태동, 송인상, 배진선, 임규, 황병두,
소속 상세정보
윤완희 ( Yoon Wan-Hee )
충남대학교 의과대학 외과학교실
최정훈 ( Choi Jung-Hoon )
충남대학교 의과대학 외과학교실
정연주 ( Jung Yeon-Joo )
충남대학교 의과대학 외과학교실
이병학 ( Lee Byung-Hak )
충남대학교 의과대학 외과학교실
김태동 ( Kim Tae-Dong )
충남대학교 의과대학 외과학교실
송인상 ( Song In-Sang )
충남대학교 의과대학 외과학교실
배진선 ( Bae Jin-Sun )
충남대학교 의과대학 외과학교실
임규 ( Im Kyu )
충남대학교 의과대학 생화학교실
황병두 ( Hwang Byung-Doo )
충남대학교 생화학교실
KMID : 0371320010610030237
Abstract
Purpose
In order to clarify the the role of epidermal growth factor (EGF) in the regulation of plasminogen activator (PA) and plasminogen activator inhibitor (PAI) during liver regeneration, we investigated the EGF-dependent gene expression of PA and
PAI-1
in
rat hepatocytes in primary culture.
Methods
Hepatocytes were isolated from rats using a two step perfusion technique and cultivated in dishes precoated with rat tail collagen. DNA synthesis of the hepatocytes by EGF treatment was measured with 3H-thymidine incorporation. Gene
expression for PAI-1, uPA and tPA was examined using Northern blot hybridization analysis.
Results
EGF treatment increased the 3H-thymidine incorporation of the hepatocytes up to 36 hours and normal polygonal hepatocyte morphology was achi
eved simultaneously. tPA and PAI-1 mRNA were detected in the control hepatocytes. With the EGF treatment, the tPA mRNA level increased with time up to 48 hours, however the PAI-1 mRNA level rapidly increased to 1 hour and then decreased quickly
to
the
control level. On the contrary, uPA mRNA was not detected in hepatocytes with or without treatment of EGF. The EGF-dependent induction of tPA and PAI-1 mRNA was a protein synthesis independent process.
Conclusion
These results suggest that differential expression of tPA and PAI-1 mRNA by EGF in hepatocytes may play an important role in the regulation of liver regeneration. Among PAs, tPA seemed to be more important in EGF dependent growth or regeneration
of
primary hepatocytes in the rat since uPA mRNA was not induced in primary hepatocyte cultures in spite of EGF treatment.
키워드
표피성장인자; 일차배양 간세포; 유전자조절; tPA; PAI-1; EGF; Primary hepatocyte; Gene regulation;
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