간암종에서 9p, 13q, 16q 염색체의 Microsatellite 변이에 대한 연구
Microsatellite Alterations of Chromosome 9p, 13q, 16q in Hepatocellular Carcinoma
조성진, 김남렬, 민연기, 조용걸, 조민영, 서성옥, 염범우, 원남희,
소속 상세정보
조성진 ( Cho Seong-Jin )
고려대학교 의과대학 병리학교실
김남렬 ( Kim Nam-Ryeol )
고려대학교 의과대학 외과학교실
민연기 ( Min Youn-Ki )
고려대학교 의과대학 외과학교실
조용걸 ( Joh Yong-Geul )
고려대학교 의과대학 외과학교실
조민영 ( Cho Min-Young )
고려대학교 의과대학 외과학교실
서성옥 ( Seo Seong-Ok )
고려대학교 의과대학 외과학교실
염범우 ( Yeom Bom-Woo )
고려대학교 의과대학 병리학교실
원남희 ( Won Nam-Hee )
고려대학교 의과대학 병리학교실
KMID : 0371320010610030305
Abstract
Purpose
Hepatocellular carcinoma (HCC) patients are asymptomatic and the tumor remains in an unresectable state until the tumor progresses. Recently much efforts for elucidation of the early hepatocarcinogenesis have been made, and for this purpose it is
very
crucial to investigate the genetic abnormalities. We evaluated microsatellite alterations of five markers from chromosome 9, 13, 16 and investigated the relationships with the clinicopathological parameters in HCC.
Methods
The microsatellite alteration analysis was performed using polymerase chain reaction with five polymorphic microsatellite markers (D9S171, D9S1747, D13S156, D16S419, D16S3106) in 40 surgically resected HC
Cs and their respective non-tumorous counterparts.
Results
D9S171, D9S1747, D13S156, D16S419, D16S3106 abnormalities were detected in 20.0%, 14.3%, 50.0%, 32.4% and 22.6%, respectively. Loss of heterozygosity (LOH) of D9S171 correlated well with higher tumor histologic grade and LOH of D13S156, D16S419
and
D16S3106 correlated well with increased tumor size. Microsatellite instability (MSI) was found in two markers, D13S156, D16S419.
Conclusion
As a result, we concluded that alterations in microsatellites of various chromosomes may contribute to the hepatocarcinogenesis and tumor progression. Especially LOH of chromosome 13 and 16 are considered to correlate with tumor progression.
키워드
간세포암종; 극소위체; 이형접합소실; Hepatocellular carcinoma; Microsatellite; Loss of heterozygosity;
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