유방암의 원발 종양 및 전이 림프절의 p53 변이양상 및 MIB-1 Index 비교
Correlation between p53 and MIB1 Index Expression of Primary Tumor and Metastatic Lymph Node in Breast Cancer
민연기, 조성진, 김남렬, 조민영, 정석인, 배정원, 원남희, 서성옥, 김영철, 구범환, 황정웅,
소속 상세정보
민연기 ( Min Youn-Ki )
고려대학교 의과대학 외과학교실
조성진 ( Cho Seong-Jin )
고려대학교 의과대학 병리학교실
김남렬 ( Kim Nam-Ryeol )
고려대학교 의과대학 외과학교실
조민영 ( Cho Min-Young )
고려대학교 의과대학 외과학교실
정석인 ( Jung Suk-In )
고려대학교 의과대학 외과학교실
배정원 ( Bae Jung-Won )
고려대학교 의과대학 외과학교실
원남희 ( Won Nam-Hee )
고려대학교 의과대학 병리학교실
서성옥 ( Seo Seong-Ok )
고려대학교 의과대학 외과학교실
김영철 ( Kim Young-Chul )
고려대학교 의과대학 외과학교실
구범환 ( Koo Bum-Hwan )
고려대학교 의과대학 외과학교실
황정웅 ( Whang Jeong-Woong )
고려대학교 의과대학 외과학교실
KMID : 0371320010610040364
Abstract
Purpose : This study was designed to elucidate the biology of cancer metastasis and differences in the biologic status between primary tumors and metastatic lymph nodes of invasive breast cancer by comparing the well known prognostic
factors
p53
gene mutation, p53 protein expression and the MIB-1 index. An additional goal was to investigate the p53 mutational pattern of breast cancer patients.
Methods : We used the PCR-SSCP method to detect p53 gene mutation and immunohistochemical staining to establish p53 protein expression and the MIB-1 labelling index in 25 primary tumors and metastatic lymph nodes from breast cancer
patients.
We
then made a comparison the between primary tumors and the metastatic lymph nodes.
Results : The results indicated a correlation between histologic grade and p53 gene mutation as well as p53 protein expression, but showed no correlation to MIB-1 labelling index. The concordance rates of p53 gene mutation and p53 protein
expression between the primary tumors and metastatic lymph nodes were 72% and 100%, respectively. Three cases showed a different mutated exon number between the primary tumors and the metastatic lymph nodes. Some cases showed p53 gene mutation
only
in
the primary tumors, but while other cases only in the metastatic lymph nodes. The MIB-1 labelling index increased with tumor grade. The p53 altered group show a higher mean MIB-1 index than the non altered group’s in both the primary tumors and
metastatic lymph nodes.
Conclusion : p53 gene mutation is not consistent with p53 protein expression and there are some differences in p53 gene mutation between primary tumors and metastatic lymph nodes in breast cancer. Therefore, metastatic tumor have different
characteristics from those of primary tumors. In breast cancer, metastasis is regulated not only by an up- regulating mechanism but also by a down-regulating mechanism.
키워드
유방암; p53 유전자 변이; p53 단백발현; MIB-1 index; Breast cancer; p53 gene mutation; p53 protein expression; MIB-1 index;
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