Gabexate Mesilate가 토끼 간의 허혈-재관류 손상에 미치는 영향
The Effects of Gabexate Mesilate on the Ishemia-Reperfusion Injury in the Rabbit Liver
신영도, 이상목, 고영관, 이기형, 고석환, 홍성화, 오수명,
소속 상세정보
신영도 ( Shin Young-Do )
경희대학교 의과대학 외과학교실
이상목 ( Lee Sang-Mok )
경희대학교 의과대학 외과학교실
고영관 ( Ko Young-Kwan )
경희대학교 의과대학 외과학교실
이기형 ( Lee Kee-Hyung )
경희대학교 의과대학 외과학교실
고석환 ( Koh Suck-Hwan )
경희대학교 의과대학 외과학교실
홍성화 ( Hong Sung-Wha )
경희대학교 의과대학 외과학교실
오수명 ( Oh Soo-Myung )
경희대학교 의과대학 외과학교실
KMID : 0371320010610060553
Abstract
Purpose : Ischemia-reperfusion is an important pathologic process that leads to impairment of the liver after major surgery. Ischmia-reperfusion injury includes both hypoxia and an inflammatory response associated with reperfusion; the
former is
caused by the lack of microvascular perfusion and the latter is mediated by cytyokines and oxygen free radicals. In addition to inhibiting thrombin, plasmin, kalikrein, trypsin, and neutrophil elastase, gabexate mesilate also plays an important
role in
inhibiting cytokines and oxygen free radical production. The purpose of this study was to investigate the effects of gabexate mesilate on ischemia-reperfusion injury in the liver.
Methods : Twenty-four New Zealand white rabbits were divided into three groups. Clamping was not done in group A (n=8), although it was done in group B (n=8) and group C (n=8). Group C received intravenous infusion of gabexate mesilate (10
㎎/㎏/hr) continuously during the process of clamping. Serum alanine aminotrasferase (ALT) and purine nucleoside phophorylase (PNP) were measured immediately before clamping, following 30-minute ischemia, and after 60-minute reperfusion. Hepatic
tissue
adenosine triphophate (ATP), xanthine oxidase, and malondialdehyde (MDA) plus 4-hydroxyalcenals (4HA) were measured after reperfusion.
Results : Compared with group A, group B and group C demonstrated a significant increase in ALT and PNP levels following ischemia and reperfusion, as well as in xanthine oxidase and MDA plus 4HA levels following reperfusion. However, ATP
levels
showed no significant differences among the three groups. ALT levels were significantly lower in group C than in group B following reperfusion (P<0.01), although there was no significant differences in PNP levels between them. Xanthine oxidase
and
MDA
plus 4HA levels were significantly lower in group C than in group B (P<0.05). The results suggest that gabexate mesilate inhibits an increase in ALT, xanthine oxidase, and MDA plus 4HA levels.
Conclusion : Gabexate mesilate inhibits oxygen free radical production of xanthine oxidase, and results in a reduction of hepatic ischemia-reperfusion injury.
키워드
Gabexate mesilate; 허혈-재관류; 간; Gabexate mesilate; Ischemia-reperfusion; Liver;
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