인간 대장암 세포주에서 Capsaicin에 의한 세포고사에 관한 연구
Study of Capsaicin-induced Apoptosis in Human Colon Cancer Cell Lines
김규열, 양경민, 성무경, 박웅채, 최대화, 남창우, 나양원, 고병균, 박건춘, 임영철, 김병삼, 조홍래,
소속 상세정보
김규열 ( Kim Kyu-Yeol )
울산대학교 의과대학 울산대학교병원 외과
양경민 ( Yang kyung-Min )
울산대학교 생명과학부
성무경 ( Seong Moo-Kyung )
건국대학교 의학전문대학원 외과학교실
박웅채 ( Park Ung-Chae )
건국대학교 의과대학 외과학교실
최대화 ( Choi Dae-Hwa )
울산대학교 의과대학 울산대학교병원 외과
남창우 ( Nam Chang-Woo )
울산대학교 의과대학 울산대학교병원 외과학교실
나양원 ( Nah Yang-Won )
울산대학교 의과대학 울산대학교병원 외과
고병균 ( Ko Byung-Kyun )
울산대학교 의과대학 울산대학교병원 외과
박건춘 ( Park Kun-Choon )
울산대학교 의과대학 울산대학교병원 외과학교실
임영철 ( Lim Young-Chul )
울산대학교 의과대학 울산대학교병원 외과학교실
김병삼 ( Kim Byung-Sam )
울산대학교 생명과학부
조홍래 ( Cho Hong-Rae )
울산대학교 의과대학 울산대학교병원 외과학교실
KMID : 0371320020620020103
Abstract
Purpose: Numerous investigations have been conducted in order to
determine the potential carcinogenic or chemopreventive activity of
capsaicin. The aim of this study is to characterize the effects of capsaicin
on colon cancer cells, and provide valuable information concerning the
application of capsaicin in chemoprevention as well as for therapeutic
purposes. Methods: CoLo320DM and LoVo cells (human colon cancer cell
line) were treated with capsaicin. In order to access cell viability and
altered morphology, an MTT assay was performed and the cells were
microscopically examined. Decreasing DNA staining was accessed by FACS. The
cells were stained with FITC labeled annexin V and analyzed by FACS to
detect cellular membrane alteration during apoptosis. The cells were stained
with DiOC6(3) and Hydroethidine and analyzed by FACS in order to access ROS
and ΔΨm. Results: Capsaicin decreased cell viability in
a dose-dependent manner. Capsaicin produced a cell morphology corresponding
to the apoptotic features including cell shrinkage and chromatic
condensation. Capsaicin treated cells induced a loss of nuclear DNA leading
to hypoploidy in a dose-dependent manner. Cells were excluded by double
staining with PI and FITC labeled annexin v and detected by FACS. We show
that treatment of CoLo320DM, L0Vo cells with increasing concentrations of
capsaicin parallel an increase in the percentage of red fluorescent cells
(HE→Eth) that reflect ROS hypergeneration and a decrease in the percentage
of green fluorescent cells that reflect ΔΨm disruption.
Conclusion: These results clearly demonstrate that capsaicin-induced
colon cancer cell death is apoptotic.
키워드
Capsaicin; 세포고사; 대장암; Capsaicin; Apoptosis; Colon cancer;
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