백서에서 수술 후 복부 유착방지용 재료들의 효능평가
Evaluation of Resorbable Materials for Preventing Surgical Adhesion on Rat Experiment
심희수, 이영우, 오영하, 권성원, 김진홍, 손승국,
소속 상세정보
심희수 ( Shim Hee-Soo )
연세대학교 의과대학 외과학교실
이영우 ( Lee Young-Woo )
한양대학교 응용화학공학부
오영하 ( Oh Young-Ha )
가천의과학대학교 해부병리학교실
권성원 ( Kwon Sung-Won )
포천중문의과대학교 외과학교실
김진홍 ( Kim Jin-Hong )
(주)바이오레인
손승국 ( Sohn Seung-Kook )
연세대학교 의과대학 외과학교실
KMID : 0371320020630030179
Abstract
Purpose: Postsurgical adhesion formation is still a cause of postoperative morbidity because no satisfactory treatment or prophylaxis has yet been developed. Therefore, we have established an animal model for adhesion prevention, and
evaluated
the toxicity, inflammation and adhesion prevention effect of available water soluble materials as barriers against adhesion formation.
Methods: Four-week old female Sprague-Dawley rats (Sam: TacN(SD)BR, Smatako Co., Seoul Korea) were used. The 2 ㎠ of cecal serosa and adjacent abdominal wall were abraded with bone burr, and the serosa of the cecum was sutured to the
abdominal
wall 1 ㎝ apart from the injured site. The denuded cecum was covered with either sodium hyaluronate (HA), sodium alginate (Alg), sodium carboxymethyl cellulose (CMC), polyethylene glycol-polypropylene glycol-polyethylene glycol (PEG-PPG,
Poloxamer), or
polyethylene glycol (PEG) on both sides, or neither side (control group) and apposed to the abdominal wall. Two weeks after operation, the quality of adhesion was graded using a whole-number system of zero to five.
Results: Adhesion grades were highest in the control group (average 4.8), were a little lower in the PEG-PPG (aver. 4.6) and PEG (aver. 4.1) groups, and were significantly lower in the HA (aver. 3.1), Alg (aver. 3.3) and CMC (aver. 3.0)
groups
(all, P＜0.05). However but inflammation was revealed in the Alg and CMC groups.
Conclusion: In our animal model for adhesion prevention, a fairly good antiadhesion effect was attained with Alg, CMC and HA, whereas inflammation was revealed with Alg and CMC. In conclusion, HA is the best candidate for adhesion
prevention.
키워드
유착; 유착방지; 수술 후 합병증; 하이알루로닉산; 동물모델; Adhesion; Adhesion prevention; Postoperative complications; Hyaluronic acid; Animal mode;
원문 및 링크아웃 정보
등재저널 정보