간세포암에서의 Genetic Instability와 5, 8, 13, 17번 염색체에서의 Microsatellite 변이에 대한 연구
Genetic Instability and Microsatellite Alterations of Chromosome 5, 8, 13, 17 in Hepatocellular Carcinoma
이경범, 최상룡, 김영철, 원남희, 서성옥, 조성진,
소속 상세정보
이경범 ( Lee Kyung-Bum )
고려대학교 의과대학 외과학교실
최상룡 ( Choi Sang-Yong )
고려대학교 의과대학 외과학교실
김영철 ( Kim Young-Chul )
고려대학교 의과대학 외과학교실
원남희 ( Won Nam-Hee )
고려대학교 의과대학 병리학교실
서성옥 ( Seo Seong-Ok )
고려대학교 의과대학 외과학교실
조성진 ( Cho Seong-Jin )
한림대학교 의과대학 병리학교실
KMID : 0371320020630030220
Abstract
Purpose: Neoplastic development is a multistep process that involves the accumulation of genetic alterations in proto- oncogenes, DNA repair genes, and tumor suppressor genes. Molecular studies in carcinoma have shown the high frequency of
loss
of heterozygosity (LOH) in some specific chromosome regions, but LOH on the HCC chromosome has not been thoroughly investigated in Korea. LOH is considered to be phenotypes of genomic instability. We investigated the genetic instability and
microsatellite alterations of chromosome 5, 8, 13 and 17 in hepatocellular carcinoma (HCC).
Methods: Microsatellite alteration analysis was performed using polymerase chain reaction with 12 polymorphic microsatellite markers (BAT26, D5S123, D5S346, D8S254, D8S261, D8S262, D13S153, D13S159, D13S171, D17S250, D17S796, TP53) in 37
surgically resected HCCs and their respective non-tumorous counterparts. Pairs of tumorous part and normal tissue in the same patient were compared and then the size of microsatellite markers was measured.
Results: MSI was detected in 3 samples and LOH was detected in 51 samples of 37 cases. Fractional allelic loss (FAL) was above 0.2 in 10 cases and was correlate with high grade of HCC. we could detect only 1 case of LOH in D8S254 marker,
which
was advanced cancer. Markers D5S123 and D5S346 showed 2 and 3 cases of LOH, respectively. Markers D8S262, D17S250 and D17S796 had LOH and were significantly correlated with tumor grade.
Conclusion: According to the results, our data revealed that specific LOH, rather than MSI, may be involved in hepatocarcinogenesis. LOH may be a useful tool for following HCC patients because the high frequency of LOH correlates with poor
prognosis of HCC.
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