위암 환자에서 RT-PCR법에 의한 복강 내 CEA 측정의 의의
Clinical Significance of Detection of Carcinoembryonic Antigen in Peritoneal Fluid by Reverse Transcription-Polymerase Chain Reaction in Patients with Gastric Cancer
인영준, 박기혁, 최동락, 주대현, 이한일, 박성환, 유용운, 전창호, 박기호,
소속 상세정보
인영준 ( In Young-Jun )
대구가톨릭대학교 의과대학 외과학교실
박기혁 ( Park Ki-Hyuk )
대구가톨릭대학교 의과대학 외과학교실
최동락 ( Choi Dong-Lak )
대구가톨릭대학교 의과대학 외과학교실
주대현 ( Joo Dae-Hyun )
대구가톨릭대학교 의과대학 외과학교실
이한일 ( Lee Han-Il )
대구가톨릭대학교 의과대학 외과학교실
박성환 ( Park Sung-Hwan )
대구가톨릭대학교 의과대학 외과학교실
유용운 ( Yoo Young-Oon )
대구가톨릭대학교 의과대학 외과학교실
전창호 ( Jeon Chang-Ho )
대구가톨릭대학교 의과대학 임상병리학교실
박기호 ( Park Ki-Ho )
대구가톨릭대학교 의과대학 외과학교실
KMID : 0371320020630040287
Abstract
Purpose: Free cancer cells in the peritoneal cavity exfoliated from a tumor are considered to be responsible for peritoneal dissemination in gastric cancer. To detect free cancer cells, a carcinoembryonic antigen (CEA) was introduced to
the
marker of gastric cancer. The clinical significance of detecting the carcinoembryonic antigen (CEA) mRNA in the peritoneal fluid was evaluated by RT-PCR in patients with gastric cancer.
Methods: In 50 patients with gastric cancer who received a gastrectomy, the peritoneal washing fluids were obtained and the CEA mRNA was detected by RT-PCR and a cytological examination was taken, simultaneously. The results were correlated
with
the stage and the recurrence of peritoneal seeding.
Results: Positive values of CEA mRNA from the peritoneal washing fluids were observed in 24% (12/50) of patients with gastric cancer but of 4% (2/50) showed peritoneal cytology. There were 8 cases of peritoneal seeding in the follow-up and
6
cases of them presented positive CEA mRNA values (50%, 6/12). According to the stage, positive CEA mRNA values from the peritoneal washing fluids were found in 9% (2/21) in stage Ⅰ, 20% (2/10) in stage Ⅱ, 33% (5/15) in stage Ⅲ and 75% (3/4) in
stage
Ⅳ (P=0.030). In the T classification, positive CEA mRNA values were found in 13% (2/15) in T1, 10% (1/10) in T2, 30% (7/23) in T3 and 100% (2/2) in T4 (P=0.031). In the N classification, positive CEA mRNA values were found in 15% (4/26) in N0,
20%
(3/15) in N1, 40% (2/5) in N2 and 75% (3/4) in N3 (P=0.055).
Conclusion: These results suggest that the detection of CEA mRNA by RT-PCR in peritoneal fluid of gastric cancer patients was more sensitive than the peritoneal cytology, and may have a role in selecting patients with a poor prognosis who
may
benefit from adjuvant therapy.
키워드
CEA mRNA; RT-PCR; 위암; CEA mRNA; RT-PCR; Stomach Ca;
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