췌장암 세포주에서의 Lewis 항원과 CA19－9 상승에 관여하는 인자 규명
Factors Related to Increased CA19-9 & Lewis Antigen in Pancreatic Cancer Cell Lines
김경식, 최진섭, 김현옥, 이우정, 김병로,
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김경식 ( Kim Kyung-Sik )
연세대학교 의과대학 외과학교실
최진섭 ( Choi Jin-Sub )
연세대학교 의과대학 외과학교실
김현옥 ( Kim Hyun-Ok )
연세대학교 의과대학 진단검사의학교실
이우정 ( Lee Woo-Jung )
연세대학교 의과대학 외과학교실
김병로 ( Kim Byong-Ro )
연세대학교 의과대학 외과학교실
KMID : 0371320020630040317
Abstract
Purpose: The 8 pancreatic cancer cell lines (BxPC-3, Capan-2, CFPAC-1, HPAC, Capan-1, AsPC-1, MIA PaCa-2, and PANC-1) were investigated to identify the factors which would increase CA19-9 related to the Lewis antigen. CA19-9 in serum is a
well-known tumor marker, and is frequently used for the clinical diagnosis of pancreatic cancer. The oligosaccharide on the CA19-9 epitope is a sialylated Lewis A blood group antigen.
Methods: β3Gal-T was detected by reverse transcriptase polymerase chain reaction (RT-PCR). The phenotypes and genotypes of Lewis antigen were determined by flow cytometry analysis and restriction fragment length polymorphism (RFLP),
respectively. The phenotypes of sLea were assessed by flow cytometry analysis and the sLea on supernatants was detected by sodium dodecyl sulfate - polyacrylamide gel electrophoresis (SDS-PAGE). CA19-9 and DUPAN-2 on
supernatants
were measured by enzyme immunoassay.
Results: CA19-9 productions were possible from all cell lines since they all had β3Gal-T and there were no genotypical Lewis negative (le/le). The elevation of CA19-9 was noted on Capan-2 and CFPAC-1, which were phenotypically Lewis
positive
(Le(a+b+)), as expected. Interestingly, it was also elevated in BxPC-3 even though the line was known to be phenotypically Lewis negative (Le(a－b－)). Sialyl Lea appeared to play an important role in this phenomenon. Although CA 19-9
was not
detected in the phenotypically Lewis negative pancreatic cell line without sialyl Lea, the levels of DUPAN-2 were variable.
Conclusion: It was revealed that an elevated CA19-9 was related with increased expression of Lewis gene, not merely the existence of the gene. Further investigations on the role of ST3Gal are warranted to explain the mechanisms of the
variable
levels of DUPAN-2 in Le(a－b－) cell lines.
키워드
Sialylation; N-acetyl glucosamine β1;3-galactosyl transferase (β3Gal-T); 루이스 항원; CA19-9; DUPAN-2; Sialylation; N-acetyl glucosamine β1;3-galactosyl transferase (β3Gal-T); α1;3/4 fucosyl transeferase (Fuc-TIII; FUT3; Lewis antigen); CA19-9; DUPAN
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