조기 위암환자에시 감시 림프절(Sentinel Node)의 탐색 및 미세 전이의 검출
Detection of Micrometastases in the Sentinel Lymph Node from Patients with Early Gastric Cancer
정명선, 김욱, 김원우, 김정수, 진형민, 박조현, 전해명, 박승만, 임근우, 김승남,
소속 상세정보
정명선 ( Jung Myung-Sun )
가톨릭대학교 의과대학 외과학교실
김욱 ( Kim Wook )
가톨릭대학교 의과대학 외과학교실
김원우 ( Kim Won-Woo )
가톨릭대학교 의과대학 외과학교실
김정수 ( Kim Jeong-Soo )
가톨릭대학교 의과대학 외과학교실
진형민 ( Chin Hyung-Min )
가톨릭대학교 의과대학 외과학교실
박조현 ( Park Cho-Hyun )
가톨릭대학교 의과대학 외과학교실
전해명 ( Jeon Hae-Myung )
가톨릭대학교 의과대학 외과학교실
박승만 ( Park Seung-Man )
가톨릭대학교 의과대학 외과학교실
임근우 ( Lim Keun-Woo )
가톨릭대학교 의과대학 외과학교실
김승남 ( Kim Seung-Nam )
가톨릭대학교 의과대학 외과학교실
KMID : 0371320020630060473
Abstract
PURPOSE: If the SLN (sentinel lymph node) is an accurate predictor of the lymph node status it should eliminate unnecessary lymph node dissection, resulting in a lower morbidity and mortality in patients with an early gastric cancer. The aim of this
study was to investigate the feasibility of SLN biopsy for its location, accuracy and ability to sensitize the tumor cell detection, using reverse transcriptase-polymerase chain reactions (RT-PCR) for the cytokeratin 19 and CD44 variants. METHODS: SLN
biopsies were performed in patients with early gastric cancer (n=33). After a gastrectomy to the apposite site, isosulfan blue dye (0.5~1.0 ml) was injected submucosal medially, laterally, superiorly and inferiorly, adjacent to the tumor site. Lymphatic
channels were immediately apparent. Sentinel nodes were separately submitted, and evaluated with RT-PCR, using the cytokeratin 19 and CD44 variants. Following the removal of the sentinel node, the adjacent regional nodes were also removed. Both SLN and
non-SLN were examined with H&E stain and RT-PCR. RESULTS: Sentinel nodes could be identified in 26 of 33 patients (79%). The average number of sentinel nodes detected was 1.4 and were chiefly perigastric nodes. Of these 26 patients, 6 had
micrometastasis in their sentinel nodes by RT-PCR detection using the cytokeratin 19 and CD44 variants. The specificity of the SLN status in the diagnosis of the lymph node status was 95% (20/21). One patient (4%) had skip metastasis. CONCLUSION: A SLN
biopsy, using indocyanine green, can be performed with a high success rate. A focused examination of the sentinel nodes detected by indocyanine green with RT-PCR will resolve the underestimation of micrometastasis in regional lymph nodes, and improve
the accuracy of the pathological staging. If the sentinel node concept is clinically feasible for early gastric cancer, we would perform not only accurate staging, but also effective surgical treatment, with minimally invasive techniques.
키워드
조기 위암; 감시 림프절; 미세 전이; Early gastric cancer; Sentinel lymph node; Micrometastases;
원문 및 링크아웃 정보
등재저널 정보