저산소-재산소화 손상에 미치는 Nitric Oxide에 대한 항산화제의 상호작용
Interactions of Nitric Oxide and Antioxidants in Hypoxia-Reoxygenation Injury
손영길, 김광일, 조해창, 이정안, 배병조, 최형철, 이광윤, 김원준,
소속 상세정보
손영길 ( Son Young-Gil )
대구파티마병원 외과
김광일 ( Kim Gwang-Il )
대구파티마병원 외과
조해창 ( Cho Hae-Chang )
대구파티마병원 외과
이정안 ( Rhee Jung-Ahn )
대구파티마병원 외과
배병조 ( Bae Byung-Jo )
대구파티마병원 외과
최형철 ( Choi Hyoung-Chul )
영남대학교 의과대학 약리학교실
이광윤 ( Lee Kwang-Youn )
영남대학교 의과대학 약리학교실
김원준 ( Kim Won-Jun )
영남대학교 의과대학 약리학교실
KMID : 0371320030640040275
Abstract
PURPOSE: An ischemia-reperfusion injury leads to profound functional and structural alterations of vascular smooth muscle cells (VSMC). It is still not clear whether hypoxia- reoxygenation and antioxidants affect the nitric oxide (NO) synthesis of VSMC. This study tried to investigate the effects of antioxidants on NO production, inducible nitric oxide synthase (iNOS) and the expression of NFκB p65, during the hypoxia-reoxygenation of VSMC cultures.
METHODS: The VSMCs were primarily cultured from rat aortae, and confirmed by immunoreaction with the anti- smooth muscle myosin antibody. The condition of the hypoxia was verified by measuring the PO(2) and PCO(2) of the culture media. The concentrations of nitrite in the culture media were measured by the Griess reaction. Western blottings for the iNOS and NFκB p65 proteins were performed. L-NAME was used as an NOS inhibitor. Vitamins C and E, Glutathione (GSH), lipoic acid and dihydrolipoic acid (DHLA) were used as antioxidants.
RESULTS: The iNOS protein was induced in the VSMC by 24 hours of hypoxia, which increased the nitrite in the VSMC culture medium. The reoxygenation profoundly increased the iNOS protein expression and nitrite concentration. The L- NAME, vitamins C and E, GSH, lipoic acid and DHLA decreased the nitrite productions during hypoxia and the hypoxia-reoxygenation, whereas, the expressions of the iNOS and NFkB p65 proteins were not influenced.
CONCLUSION: We concluded that hypoxia-reoxygenation induced the iNOS protein, and the subsequent production of NO in the VSMC. The antioxidants and the NOS inhibitor decreased the NO production during the hypoxia-reoxygenation, but did not affect the expressions of the iNOS and NFκB p65 proteins.
키워드
일산화질소;저산소-재산소화;항산화제
Nitric oxide;Hypoxia-reoxygenation;Antioxidant
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