마우스 간 수상세포에 의해 유도된 활성화 T 임파구 세포사멸과 그 기전에 관한 연구
A Study for Apoptosis and Its Mechanism of Allogeneic Activated T Lymphocytes Induced by Mouse Liver Immature Dendritic Cells
이영철, 김정진, 박혜림, 이삼열, 우영민, 조마해, 김주섭, 이봉화,
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이영철 ( Lee Young-Cheol )
한림대학교 의과대학 외과학교실
김정진 ( Kim Jeong-Jin )
한림대학교 의과대학 외과학교실
박혜림 ( Park Hye-Rim )
한림대학교 의과대학 병리학교실
이삼열 ( Lee Sam-Uel )
한림대학교 의과대학 외과학교실
우영민 ( Woo Young-Min )
한림대학교 의과대학 일반외과학교실
조마해 ( Cho Ma-Hae )
한림대학교 의과대학 외과학교실
김주섭 ( Kim Joo-Seop )
한림대학교 의과대학 외과학교실
이봉화 ( Lee Bong-Wha )
한림대학교 의과대학 외과학교실
KMID : 0371320040660010001
Abstract
Purpose: Mouse liver nonparenchymal cells play an important role n the development of active apoptosis in graftinfiltrating cytotoxic T lymphocytes, and this apoptosis can be an explanation for liver graft acceptance. We intended to clarify whether immature mouse liver dendritic cells can induce apoptosis in allogeneic activated T cells and determine which mechanism is involved in this phenomenon.
Methods: A radiometric DNA fragmentation test ("JAM" assay) was used to determine whether mouse liver dendritic cells were eble to induce activated T-cell apoptosis in vitro. In addition, immunohistochemical staining for Bax and Bcl-2 was examined to clarify whether Bax or Bcl-2 was involved in this apoptosis.
Results: Immature mouse liver dendritic cells were quite strong inducers of activated T cell apoptotic death in allogeneic mice in vitro (39.2±13.2% at E/T ratio=12.5/1) compared with spleen cells as effectors (4.7±13.4% at E/T ratio=12.5/1) (P<0.0001). By using immunohistochemical staining, we also showed that Bax might play some role in this phenonenon, but that Bcl-2 might not.
Conclusion: Our data indicate that immature mouse liver dendritic cells might have a strong apoptotic activity toward activated T cells in allogeneic mice in vitro through a Bax involved mechanism.
키워드
간 수상세포;T 임파구;Bax;Liver dendritic cells;T cell apoptosis
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