MIP-1 Alpha가 CD4^(＋) Th 림프구에 미치는 영향
Influence of MIP-1 Alpha on the CD4^(＋) Th Lymphocytes
박종권, 김재경,
소속 상세정보
박종권 ( Park Jong-Kwon )
단국대학교 의과대학 외과학교실
김재경 ( Kim Jae-Kyung )
단국대학교 의과대학 진단검사의학과
KMID : 0371320040660020081
Abstract
Purpose: MIF-1 alpha, initially identified as a substance to promote inflammation, was recently discovered to also suppress prolferation of many kinds of cells, In this study, the influence of MIP-1 alpha on CD4^(+) Th cells to secrete cytokines (IL-10, IL-2, IFN-gamma) and to express CD25 molecules was investigated.
Methods: Peripheral blood mononuclear cells (PBMCs) were prepared from five normal volunteers and initially divided into 4 groups: IL-10, IL-2, IFN-gamma, and CD25. Each group was further divided into 4 subgroups according to the incubation with or without MIP-1 alpha and to the incubation for 30 minutes or 3 hours. Analysis was performed by flow cytometry.
Results: Incubation, of CD4^(+) Th lymphocytes with MIP-1 alpha showed a tendency to increase Th1 cytokine (IL-2, IFN-gamma) secretion and to decrease Th2 cytokine (IL-10) release, but there was no significant difference between any of the experimental groups, Among the CD4^(+) Th lymphocyte groups cultured with MIP-1 alpha, the expression of CD25 was significantly lower in the 3-hour incubation group than in the 30-minute group (P=0.008).
Conclusion: MIP-1 alpha may play a role in facilitating immune response by increasing Th1 and decreasing Th2 cytokine secetion from CD4^(+) Th cells, and also by decreasing the proportion of CD4^(+) CD25^(+) Th cells in the peripheral blood However, in vivo study is necessary to verify the function of MIP-1 alpha in the living body.
키워드
Th 림프구;말초혈액단행세포;면역기능;MIP-1 alpha;CD^(+) Th lymphocyte;CD25
원문 및 링크아웃 정보
등재저널 정보