Nitric Oxide Donor가 신장 허혈 재관류 손상 시 내인성 Endothelin-1 분비에 미치는 영향
The Effect of Nitric Oxide Donor Endogenous Endothelin-1 Expression in Renal Ischemia-Reperfusion Injury
정규영, 성순희, 김상이, 손정은, 이우정, 김유선, 정구용,
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정규영 ( Jung Gyu-Young )
이화여자대학교 의과대학 외과학교실
성순희 ( Sung Soon-Hee )
이화여자대학교 의과대학 해부병리학교실
김상이 ( Kim Sang-Yee )
이화여자대학교 의과대학 외과학교실
손정은 ( Sohn Jeong-Eun )
이화여자대학교 의과대학 외과학교실
이우정 ( Lee Woo-Jung )
연세대학교 의과대학 외과학교실
김유선 ( Kim Yu-Seun )
연세대학교 의과대학 외과학교실
정구용 ( Chung Ku-Yong )
이화여자대학교 의과대학 외과학교실
KMID : 0371320040660030169
Abstract
Purpose: The balance between nitric oxide (NO) and endothelin-1 (ET-1 production is essential to vascular function in controlling organ perfusion, and an elevated ET-1 in the peritubular capillary network, following renal transplantation, can be associated with renal allograft rejection. The administration of a nitric oxide donor during the preischemic period has been shown to protect the kidneys against an ischemia-reperfusion injury, but the mechanism underlying this therapeutic benefit remains to be completely understood. Our hypothesis is that the early administration of the NO donor, sodium nitroprusside (SNP), may suppress ET-1, and thereby improve the renal function in an ischemia-reperfusion injury.
Methods: Sprague Dawley rats were subjected to 60 minutes of renal warm ischemia, followed by a contralateral nephrectomy. Renal biopsies were performed prior to ischemia and reperfusion, and at 1 and 48 hours after the reperfusion. The animals were divided into 4 groups: a sham group, without warm ischemia, an early SNP group (SNP given before schemia), a late SNP group (SNP given before reperfusion) and an ischemic control group. The ET-1 expression was assessed by a semiquantitative analysis by immunohistochemical staining with the ET-1 monoclonal antibody and Hematoxylin-Eosin stain. The serum creatinine was measured at 48 hours after the reperfusion.
Results: There were significant improvements in all parameters of the early SNP group compared with those in the late SNP and ischemic control groups, but there was no difference between the late SNP and ischemic control groups.
Conclusion: These data suggest that the early administration of SNP in renal ischemia-reperfusion improves the renal function by suppressing the expression of ET-1.
키워드
엔도텔린;나이트릭옥사이드;신장허혈 재관류손상;Endothelin-1;Nitric oxide;Renal ischemia-re-perfusion
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