대장암에서 CK20과 CEA의 RT-PCR을 이용한 수술조작 전후 유출 정맥과 복막에서의 미세전이의 검출
Detection of Micrometastasis in Draining Vein before & after Manipulation and Peritoneal Cavity in Colorectal Cancer by Reverse Transcriptase-Polymerase Chain Reaction for Cytokeratin 20 and Carcinoembryonic Antigen
주재균, 이지희, 박영규, 류성엽, 김동의, 김영진,
소속 상세정보
주재균 ( Ju Jae-Kyun )
전남대학교 의과대학 외과학교실
이지희 ( Lee Ji-Hee )
전남대학교 의과대학 외과학교실
박영규 ( Park Young-Kyu )
전남대학교 의과대학 외과학교실
류성엽 ( Ryu Seong-Yeop )
전남대학교 의과대학 외과학교실
김동의 ( Kim Dong-Yi )
전남대학교 의과대학 외과학교실
김영진 ( Kim Young-Jin )
전남대학교 의과대학 외과학교실
KMID : 0371320040660030194
Abstract
Purpose: Micrometastasis is known as a significant predictor of prognosis in colorectal cancer patients. Recently, reverse transcriptase polymerase chain reaction (RT-PCR) has been applied to detecting micrometastasis. The drainage vein and peritoneum were examined and the micrometastases assessed in a series of colorectal cancer patients.
Methods: 22 patients, who were histologically diagnosed with colorectal cancer, and 8 patients of serosal and peritoreal brushing, were examined using RT-PCR to amplify the mRNAs for two epithelial markers, carcinoembryonic antigen (CEA) and cytokeratin 20 (CK-20).
Results: Among the 22 colorectal cancer patients, the positive rates of CK-20 and CEA mRNAs in the drainage vein were 10 (45%) and 7 (32%), and those of the serosal and peritoneal brushing were 6 (75%) and 5 (63%), respectively.
Conclusion: These results suggest that the "no touch isolation technique" might be useful for operations in advanced coloretal cancer patients, and the brushing of the serosal or Douglas pouch can represent the micro metastasis status.
키워드
대장암;미세전이;역전사-중합효소연쇄반응;암태아성 항원;CK-20;Colorecral cancer;Micrometastasis;Reverse transcriptase polymerase chain reaction;RT-PCR;carcinoembryonic antigen;CEA;Cytokeratin 20
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